Name: X-GlcA
Brand: SIGMA

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About This Item

Empirical Formula (Hill Notation):

C₁₄H₁₃BrClNO₇ · C₆H₁₃N

CAS Number:

114162-64-0

Molecular Weight:

521.79

MDL number:

MFCD00058543

UNSPSC Code:

12352200

PubChem Substance ID:

24891926

NACRES:

NA.85

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grade

Molecular Biology

sterility

non-sterile

assay

≥98% (TLC)

form

powder

technique(s)

nucleic acid detection: suitable

solubility

DMF: soluble

suitability

suitable for β-galactosidase test

storage temp.

−20°C

SMILES string

NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34

InChI

1S/C14H13BrClNO7.C6H13N/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2/t9-,10-,11+,12-,14+;/m0./s1

InChI key

JXCKZXHCJOVIAV-CYRSAHDMSA-N

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Cloning & Expression

Molecular Cloning & Protein Expression

General description

X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Chromogenic substrate for β-glucuronidase (GUS) gene detection.

Suitable for use in selection of recombinant bacterial colonies with the lac⁺ phenotype.
X-GlcA has been used in histochemical staining of root sections for light microscopic observation.

Principle

When X-GlcA is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GlcA turn an intense blue color, allowing for easy differentiation between lac+ and lac- colonies.

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