MilliporeSigma
All Photos(2)

Documents

B5285

Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt

≥98%

Sign Into View Organizational & Contract Pricing

Synonym(s):
BC-Indicator, X-GlcA, X-glucuronide
Empirical Formula (Hill Notation):
C14H12BrClNNaO7
CAS Number:
Molecular Weight:
444.59
MDL number:
PubChem Substance ID:

Assay

≥98%

form

powder

solubility

water: 50 mg/mL, clear, colorless

storage temp.

−20°C

SMILES string

[Na+].O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C([O-])=O)Oc2c[nH]c3ccc(Br)c(Cl)c23

InChI

1S/C14H13BrClNO7.Na/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;/h1-3,9-12,14,17-20H,(H,21,22);/q;+1/p-1/t9-,10-,11+,12-,14+;/m0./s1

InChI key

IBLSVGDGSKUDCT-CYRSAHDMSA-M

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
B8174B4532B4527
form

powder

form

tablet

form

powder

form

powder

solubility

water: 50 mg/mL, clear, colorless

solubility

deionized water: one tablet, 5 mg/mL

solubility

-

solubility

DMF: 50 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt has been used:
  • as a component in β-glucuronidase (GUS) assay buffer for histochemical GUS assay
  • to prepare X-Gluc or GUS staining solution
  • to confirm GUS expression in samples by X-gluc staining

Chromogenic substrate for β-glucuronidase (GUS) gene detection.

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA), a colorless substrate for β-glucuronidase enzyme, generates a blue color at the end of the reaction.

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Agrobacterium tumefaciens-mediated Transformation of Walnut (Juglans regia)
Walawage SL, et al.
Plant Physiology (2014)
Multifunctional Paper-Based Analytical Device for In Situ Cultivation and Screening of Escherichia coli Infections
Noiphung J and Laiwattanapaisal W
Scientific reports, 9(1), 1555-1555 (2019)
GUS Staining of Guard Cells to Identify Localised Guard Cell Gene Expression
Liu Z, et al.
Plant Physiology (2017)
Development of an efficient bi-directional promoter with tripartite enhancer employing three viral promoters
Patro S, et al.
Journal of Biotechnology, 163(3), 311- 317 (2013)
Grégory Montiel et al.
Plants (Basel, Switzerland), 9(4) (2020-04-08)
Until recently, the roles of plant MADS-box genes have mainly been characterized during inflorescence and flower differentiation. In order to precise the roles of AGAMOUS-LIKE 12, one of the few MADS-box genes preferentially expressed in roots, we placed its cDNA

Articles

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service