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71146

Sigma-Aldrich

pETDuet-1 DNA - Novagen

pETDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites, each of which is preceded by a T7 promoter, lac operator and ribosome binding sites.

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Quality Level

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

promoter

Promoter name: T7

selection

ampicillin

shipped in

wet ice

storage temp.

−70°C

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pETDuet-1 DNA - Novagen pETDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites, each of which is preceded by a T7 promoter, lac operator and ribosome binding sites.

71146

pETDuet-1 DNA - Novagen

pACYCDuet-1 DNA - Novagen pACYCDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is receded by a T7 promoter, lac operator and ribosome binding site.

71147

pACYCDuet-1 DNA - Novagen

pRSFDuet-1 DNA - Novagen

71341

pRSFDuet-1 DNA - Novagen

Sigma-Aldrich

70824

pTriEx-4 DNA - Novagen

form

liquid

form

liquid

form

liquid

form

liquid

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−20°C

tag

His tagged

tag

His tagged, S-Tag tagged

tag

-

tag

-

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

General description

Coexpression of multiple target genes in E. coli is advantageous for studying protein complexes. Coexpression often achieves optimal yield, solubility, and activity and may protect individual subunits from degradation. The Duet vectors are T7 promoter expression vectors, each designed to coexpress two target proteins in E. coli. The Duet vectorscarry compatible replicons and antibiotic resistance markers and may be used together in appropriate host strains to coexpress up to eight proteins. Certain combinations of Duet vectors and pET or pETcoco vectors are also compatible for coexpression. The capability of Duet vectors to be cotransformed, propagated, and induced for robust target proteincoexpression makes them ideal for the analysis of protein complexes. The Duet vectors are designed with compatible replicons and drug resistance genes for effective propagation and maintenance of four plasmids in a single cell.
pETDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS), each of which is preceded by a T7 promoter, lac operator and ribosome binding site (rbs). The vector also carries the pBR322-derived ColE1 replicon, lacI gene and ampicillin resistance gene. This vector can be transformed into the same cell with pACYCDuet-1, pCDFDuet-1, and pRSFDuet-1 or pCOLADuet-1 in the appropriate host strain for the coexpression of up to eight target genes. Genes inserted into MCS-1 can be sequenced using the pETUpstream Primer and DuetDOWN1 Primer. Genes inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.




Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.

Warning

Toxicity: Standard Handling (A)

Other Notes

Vector Family: Duet pET bacterial prokaryotic

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Yang Zhang et al.
Journal of molecular biology, 431(24), 4882-4896 (2019-11-05)
Multidrug-resistant gram-negative bacteria infection is particularly severe within the designated ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species), which underscores the urgent need to explore alternative therapeutic strategies. The type III secretion system

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