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SH2121

Sigma-Aldrich

MISSION® shRNA Human Gene Family Set, DNA

Ubiquitin Ligases (E1,E2,E3)

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200 PKG
$944.00

$944.00

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200 PKG
$944.00

About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

$944.00

Check Cart for Availability
For a suitable alternative that ships today, try SLHVMZ5NZ.
Request a Bulk OrderRequest more information

Quality Level

200

product line

MISSION®

storage temp.

−20°C

Gene Information

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This Item
SLHNM25SLGNM25SLHNBZ5NK
Millex™ Nylon syringe filter pore size 0.45 μm, diam. 25 mm, non-sterile, hydrophilic, glass fiber prefilter

SLHNMZ5NZ

Millex™ Nylon syringe filter

Millex™ Nylon syringe filter pore size 0.45 μm, diam. 25 mm, non-sterile, hydrophilic, glass fiber prefilter

SLHNM25

Millex™ Nylon syringe filter

Millex™ Nylon syringe filter pore size 0.2 μm, diam. 25 mm, non-sterile, hydrophilic, glass fiber prefilter

SLGNM25

Millex™ Nylon syringe filter

Millex™ Nylon syringe filter pore size 0.45 μm, diam. 25 mm, non-sterile, hydrophilic, glass fiber prefilter

SLHNBZ5NK

Millex™ Nylon syringe filter

diam.

25 mm

diam.

25 mm

diam.

25 mm

diam.

25 mm

material

Nylon membrane, high-density polyethylene housing, glass fiber prefilter

material

Nylon membrane, glass fiber prefilter, high-density polyethylene housing

material

Nylon membrane, glass fiber prefilter, high-density polyethylene housing

material

Nylon membrane, glass fiber prefilter, high-density polyethylene housing

pore size

0.45 μm

pore size

0.45 μm

pore size

0.2 μm

pore size

0.45 μm

manufacturer/tradename

Millex™

manufacturer/tradename

Millex™

manufacturer/tradename

Millex™

manufacturer/tradename

Millex™

fitting

female Luer-Lok® inlet, male Luer outlet slip

fitting

female Luer-Lok® inlet, male Luer outlet slip

fitting

female Luer-Lok® inlet, male Luer outlet slip

fitting

female Luer-Lok® inlet, male Luer outlet slip

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

General description

Sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) are provided as purified plasmid DNA suitable for virus production and transient transfection. DNA is provided in 40 μl aliquots per well in Tris,EDTA (TE) solution. An average of 2 μg per clone is provided per well with a range from 400 ng to 4 μg. The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

Other Notes

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 341, Number of Clones: 2081
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Kim Newton et al.
    Cancer investigation, 25(6), 502-513 (2007-09-21)
    The regulated degradation of cellular proteins by the ubiquitin-proteasome system impacts a range of vital cellular processes in both normal and cancerous cells. An ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2), and ubiquitin ligase (E3) catalyzes the conjugation of the protein
    Timothy Cardozo et al.
    BMC biochemistry, 8 Suppl 1, S9-S9 (2007-12-06)
    Recently, the ubiquitin proteasome system (UPS) has matured as a drug discovery arena, largely on the strength of the proven clinical activity of the proteasome inhibitor Velcade in multiple myeloma. Ubiquitin ligases tag cellular proteins, such as oncogenes and tumor

    Articles

    Efficient Gene Silencing with MISSION® TRC shRNA

    RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service