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SH2621

Sigma-Aldrich

MISSION® shRNA Human Gene Family Set, DNA

Helicases

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51
Pricing and availability is not currently available.

Quality Level

200

product line

MISSION®

storage temp.

−20°C

Gene Information

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grade

certified reference material, pharmaceutical secondary standard

grade

certified reference material, pharmaceutical secondary standard

grade

certified reference material, pharmaceutical secondary standard

grade

certified reference material, pharmaceutical secondary standard

application(s)

pharmaceutical

application(s)

pharmaceutical small molecule

application(s)

pharmaceutical

application(s)

pharmaceutical

CofA

current certificate can be downloaded

CofA

-

CofA

current certificate can be downloaded

CofA

current certificate can be downloaded

packaging

pkg of 500 mg

packaging

pkg of 100 mg

packaging

pkg of 500 mg

packaging

pkg of 500 mg

Quality Level

300

Quality Level

-

Quality Level

-

Quality Level

300

General description

Sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) are provided as purified plasmid DNA suitable for virus production and transient transfection. DNA is provided in 40 μl aliquots per well in Tris,EDTA (TE) solution. An average of 2 μg per clone is provided per well with a range from 400 ng to 4 μg. The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

Other Notes

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 136, Number of Clones: 909
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Taekjip Ha
    Cell, 129(7), 1249-1250 (2007-07-03)
    There is much debate about how helicases unwind DNA during DNA replication and how their activity is regulated. In this issue, Johnson et al. (2007) shed light on this conundrum using a single molecule approach to dissect the behavior of
    Xu Guang Xi
    Current medicinal chemistry, 14(8), 883-915 (2007-04-14)
    In the cell, the unwinding of double-stranded polynucleotides is catalyzed by helicases that are present in all kingdoms of life from virus to man. Viruses, like all other organisms, synthesize their DNA or RNA genomes in a template-dependent manner. In

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