D7295

Deoxynucleotide Mix, 10 mM

Molecular Biology Reagent

• Synonyma: Deoxynucleotide Mix, 10 mM, 10mM dNTP mix, dNTP mix, dNTPs
• NACRES: NA.52
• UNSPSC Code: 41106305
• Assay: ≥99%
• Biological source: synthetic (organic)
• Form: liquid
• Solubility: H₂O: soluble at 20 °C
• Storage temp.: −20°C

Vlastnosti

• Název produktu: Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent
• biological source: synthetic (organic)
• assay: ≥99%
• form: liquid
• concentration: 10 mM
• color: colorless
• solubility: H₂O: soluble at 20 °C
• application(s): agriculture
• foreign activity: DNase, RNase, none detected
• shipped in: dry ice
• storage temp.: −20°C
• Quality Level: 300

Popis

Application

dNTP Mix has been used:

• in the PCR amplification of genomic DNA isolated from insect, fungi, virus, human,
• in reverse transcription of total RNA to cDNA.
• as a component of the DNA amplification mixture for polymerase chain reaction (PCR)
• routine and long PCR
• manual and automated DNA sequencing
• cDNA synthesis and labeling reactions

Features and Benefits

• Purity of each dNTP: Minimum 99%
• Conveniently formulated; 1 μL is used per 50 μL PCR
• Equimolar amounts of each dNTP means less pipetting
• Minimize risk of contamination in PCR
• UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions

General description

Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide. The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water. One µL is sufficient for a standard 50 µL PCR reaction.

Bezpečnostní informace

• Skladovací třída: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable