Skip to Content
Merck
All Photos(6)

Key Documents

View All Documentation

XTG9-RO

Roche

X-tremeGENE™ 9 DNA Transfection Reagent

Polymer reagent for transfecting common cell lines

Sign Into View Organizational & Contract Pricing
All Photos(6)

About This Item

UNSPSC Code:
41106502
NACRES:
NA.55

grade

for molecular biology

Quality Level

100

form

liquid (aqueous solution)

usage

 mL (suitable for 165 transfections)

packaging

pkg of 0.4 mL (06365779001)
pkg of 1.0 mL (06365787001)
pkg of 5 × 1.0 mL (06365809001)

manufacturer/tradename

Roche

technique(s)

transfection: suitable

storage temp.

2-8°C

Related Categories

General description

X-tremeGENE 9 DNA Transfection Reagent is a proprietary blend of lipids and other components supplied in 80% ethanol, filtered through 0.2 μm pore size membrane, and packaged in glass vials.

Application

X-tremeGENE™ 9 DNA Transfection Reagent is a non-liposomal multi-component reagent for experiments involving cellular analysis. Due to its extremely low cytotoxicity, minimal need for optimization, and the ability to provide high transfection efficiency in a wide range of commonly used cell lines even in the presence of serum, it is well suited for applications in all fields of cellular analysis.

X-tremeGENE™ 9 DNA Transfection Reagent is well suited for cellular analysis applications such as:
  • Expression of recombinant proteins for functional analysis.
  • Physiological studies of metabolic pathways.
  • Analysis of regulatory sequences using reporter gene assays.
  • Gene expression assays.
  • Cancer research studies.
  • Target evaluation.

Features and Benefits

  • Generate physiologically relevant results using a reagent with extremely low cytotoxicity for maximum post-transfection cell viability.
  • Save time and eliminate multiple handling steps; simply dilute X-tremeGENE 9 DNA Transfection Reagent, incubate with plasmid DNA, and pipet the mixture directly onto your cells (with or without serum).
  • Avoid time-consuming optimization procedures in commonly used cell lines.

Quality

Each lot of X-tremeGENE™ 9 DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE™ 9 DNA Transfection Reagent (ratio 3:1 μl/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE 9 DNA Transfection Reagent can be used to perform up to 6,600 transfections in 96-well plates using 3:1 ratio and up to 10,000 transfections using 2:1 ratio.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

X-tremeGENE is a trademark of Roche

related product

Product No.
Description
Pricing

D5796

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

R8758

RPMI-1640 Medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

NA0300

GenElute™ HP Plasmid Maxiprep Kit, sufficient for 10 purifications

NA0400

GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit, sufficient for 10 preparations

PP2393

Mammalian FLAG® Tag Vector Set, plasmid vectors for molecular cloning

XTGHP-RO

X-tremeGENE™ HP DNA Transfection Reagent, High-performance polymer reagent for transfecting many cell lines

SITRAN-RO

X-tremeGENE™ siRNA Transfection Reagent, Polymer reagent for delivering siRNA to common cell lines

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225 - H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

334.4 °F

Flash Point(C)

168 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Daniel F Comiskey et al.
Nucleic acids research, 43(8), 4202-4218 (2015-04-08)
Genotoxic stress induces alternative splicing of the oncogene MDM2 generating MDM2-ALT1, an isoform attributed with tumorigenic properties. However, the mechanisms underlying this event remain unclear. Here we explore MDM2 splicing regulation by utilizing a novel minigene that mimics endogenous MDM2
Birte Zurek et al.
Methods in molecular biology (Clifton, N.J.), 748, 107-119 (2011-06-28)
Nod1 and Nod2 are pattern recognition receptors of the mammalian innate immune system. They respond to bacterial peptidoglycan fragments and are implicated in host defense against a variety of -different bacterial pathogens. Recent studies furthermore support additional functions of these
Jean Guillon et al.
Journal of enzyme inhibition and medicinal chemistry, 18(2), 147-153 (2003-08-29)
The syntthesis of new N-propargyl-3-pyrrol-1-ylindanamine derivatives, analogues of rasagiline, is described in ten steps starting from the corresponding arylaldehydes via the corresponding cis-3-pyrrol-1-ylindanamines. The cis-configuration of some intermediates has been established using X-ray analysis and NOE experiments. The new N-propargyl-3-pyrrol-1-ylindanamine
L C Costantini et al.
Neuroscience, 89(2), 505-513 (1999-03-17)
To investigate the role of neurotrophins in the initial formation of striatal patch versus matrix, the spatial and temporal expression of trkB receptors was examined using immunohistochemistry. Polyclonal antibodies, against the C-terminus or the tyrosine kinase domain, revealed trkB-immunoreactive cells
Leonardo Freire-de-Lima et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(43), 17690-17695 (2011-10-19)
The process termed "epithelial-mesenchymal transition" (EMT) was originally discovered in ontogenic development, and has been shown to be one of the key steps in tumor cell progression and metastasis. Recently, we showed that the expression of some glycosphingolipids (GSLs) is
View All Related Papers

Articles

Reverse Transfection of Plasmid DNA

Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

Protocols

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service