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72181

Sigma-Aldrich

293-Free Transfection Reagent

Animal-free polycationic liposomal transfection reagent optimized for the transfection of HEK293 cells grown in suspension culture.

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Synonym(s):
Gene delivery

Quality Level

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

technique(s)

transfection: suitable

shipped in

wet ice

storage temp.

2-8°C

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This Item
71115SITRAN-RO71259
technique(s)

transfection: suitable

technique(s)

transfection: suitable

technique(s)

transfection: suitable

technique(s)

transfection: suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Roche

manufacturer/tradename

Novagen®

storage condition

OK to freeze

storage condition

do not freeze

storage condition

-

storage condition

OK to freeze

General description

Animal-free polycationic liposomal transfection reagent optimized for the transfection of HEK293 cells grown in suspension culture.
Human Embryonic Kidney 293 cells, often referred to as HEK 293, HEK-293, or 293 cells, are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture. HEK293 cells grow robustly and are often transfected with DNA plasmids for many cell biology research applications. HEK293 cells are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy. 293-Free<TMSYMBOL></TMSYMBOL> Transfection Reagent has a unique polycationic liposomal formulation and is designed expressly for transfection of HEK293 cells grown in suspension culture. It is ideal for mammalian protein production. Derived from non-animal sources, 293-Free Transfection Reagent gives minimal cellular toxicity. It is provided as a sterile, ready-to use solution. The 1 ml size provides enough reagent to transfect a liter of culture.

Features and Benefits

  • Excellent transfection efficiency for HEK293 suspension cultures for maximize protein expression levels
  • Derived from non-animal sources to move faster from research to development
  • Compatible with both serum-containing and serum-free media that simplifies protocol by eliminating media changes
  • Minimal cellular toxicity leads to higher protein expression levels

Warning

Toxicity: Flammable (J)

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

67.1 °F - Information taken from reference works and the literature.

Flash Point(C)

19.5 °C - Information taken from reference works and the literature.


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