Transfer Membranes & Kits
Immobilon® PVDF and nitrocellulose transfer membranes provide high sensitivity and low background in Western blotting and other blotting applications. Tailored for specific applications, detection chemistries, and blotting techniques, Immobilon® membranes are offered in a variety of options for optimal results, flexibility, and convenience.
We offer four types of Immobilon® PVDF membranes as well as Immobilon® nitrocellulose membranes, each optimized for a specific protein blotting application. Blotting sandwiches that feature pre-cut sheets of membrane and blotting filter paper are also available.
- Immobilon® NC membrane is made of nitrocellulose, exhibiting lowest background due to decreased non-specific antibody binding with the ability to bind both proteins and nucleic acids for a variety of blotting applications.
- Immobilon®-E membrane (0.45 µm) is the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step. This membrane is well-suited for most Western blotting applications.
- Immobilon®-P membrane (0.45 µm) is optimized for Western blotting and offer better handling and staining than nitrocellulose. All Immobilon®-P PVDF membranes have a pore size of 0.45 μm.
- Immobilon®-PSQ membrane (0.2 µm) is ideal for protein sequencing and immunoblotting of low molecular weight proteins. It has a higher protein binding capacity and a higher retention than 0.45 µm membranes.
- Immobilon®-FL membrane (0.45 µm) was developed for fluorescence-based immunodetection. It has low background fluorescence across a wide range of excitation and emission wavelengths.
- Immobilon® NOW rolls (8.5cm x 10m) provide improved convenience over standard transfer membrane rolls. Get a standard mini or mid blot with a single cut using measurement marks on the package lid.
Products
Immobilon® Family of Transfer Membranes | ||||||
|---|---|---|---|---|---|---|
| Membrane Name | Immobilon® -NC | Immobilon® -E | Immobilon® -P | Immobilon® -FL | Immobilon® -PSQ | |
Chemistry | Nitrocellulose and MCE | PVDF | PVDF | PVDF | PVDF | |
Pore Size | 0.45 µm | 0.45 µm | 0.45 µm | 0.45 µm | 0.2 µm | |
Detection Methods | Chemiluminescence, Colorimetric | Chemiluminescence, Colorimetric | Chemiluminescence, Colorimetric | Chemiluminescence, Colorimetric, Fluorescence (including near-infrared) | Chemiluminescence, Colorimetric | |
Binding Capacity | Goat IgG | 237 µg/ cm2 | 225-293 μg/cm2 | 294 μg/cm2 | 300 μg/cm2 | 448 μg/cm2 |
BSA | N/A | N/A | 215 μg/cm2 | 205 μg/cm2 | 340 μg/cm2 | |
Insulin | N/A | N/A | 160 μg/cm2 | 155 μg/cm2 | 262 μg/cm2 | |
Applications | Western, Southern and Northern blots, dot blots | Western blots | Western blots, dot blots | Western blots, dot blots | Western blots, dot blots | |
The durability and high protein retention of Immobilon® PVDF membranes make them the preferred option for sequential detection of multiple targets via antibody stripping and reprobing. | ||||||
Selection Criteria | •High-abundance target protein •Wide range of molecular weights | •Target protein expressed over a broad dynamic range •Wide range of molecular weights | •Target protein expressed over a broad dynamic range •Wide range of molecular weights | •Target protein expressed over a broad dynamic range •Wide range of molecular weights | •Target protein <20 kDa •Very low abundance target protein •Low affinity antibody | |
Immobilon® Nitrocellulose Membranes for Western Blotting
Nitrocellulose (NC) membranes bind proteins through hydrophobic interactions. Immobilon® nitrocellulose membranes:
- Bind both proteins and nucleic acids
- Compatible with Western blotting, Northern blotting, Southern blotting, and dot blot techniques
- Exhibit low background attributed to decreased non-specific antibody binding
- Demonstrate excellent binding capacity for low molecular weight proteins
- Optimized for chemiluminescent, chromogenic, and fluorescent detection
Related Product Resources
- Article: Methods Used to Detect Proteins and Nucleic Acids Bound to Membranes
Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
- Brochure: Rethink Western Blotting
Explore our products designed to improve each step of the Western blotting workflow.
- Handbook: Protein Blotting Tips and Tricks
This handbook represents the collective experience of our application scientists, who are actively engaged in advancing the science of protein blotting and detection.
- Article: Western Blotting Protocol (Immunoblotting Protocol)
Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.
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