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IPFL00005

Millipore

Immobilon® -FL PVDF Membrane

1 roll, 27 cm x 1.875 m, 0.45 µm pore size, transfer membrane with low background fluorescence

Synonym(s):

Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.23

product name

Immobilon®-FL PVDF Membrane, 1 roll, 27 cm x 1.875 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

material

PVDF membrane
plain filter
white filter

Quality Level

400

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

27 cm × 1.875 m

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin)
 205 μg/cm2 adsorption capacity (BSA)
 300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with All fluorescence dyes
for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby

detection method

chemiluminescent
colorimetric
fluorometric

shipped in

ambient

Application

Immobilon®-FL PVDF membrane has been used in western blotting.

Features and Benefits

Immobilon®-FL PVDF membrane is compatible with the following stains:
  • All fluorescence dyes
  • Coomassie brilliant blue
  • Amido black
  • Ponceau-S red
  • CPTS
  • Sypro® ruby

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
SYPRO is a registered trademark of Life Technologies

Storage Class

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Semi-Dry or Tank Electrotransfer Troubleshooting

Troubleshoot protein transfer issues in Western blotting by understanding causes and optimizing transfer conditions.

Protocols

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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