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A0521

Sigma-Aldrich

α-Amylase from human saliva

greener alternative

Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein

Synonym(s):

1,4-α-D-Glucan-glucanohydrolase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

type

Type IX-A

Quality Level

form

lyophilized powder

specific activity

1,000-3,000 units/mg protein

composition

Protein, 5.0-15.0% biuret

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Waste Prevention
Design for Energy Efficiency
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UniProt accession no.

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storage temp.

−20°C

Gene Information

human ... AMY1A(276)

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General description

α-amylase is the main secretory protein constituent of saliva. It contains 496 amino acids and has two isoforms, glycosylated and non-glycosylated. α-amylase gene is located on human chromosome 1p21.1. α-amylase is mainly produced in pancreas and saliva.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Application

α-amylase from human saliva has been used to test α-amylase inhibitors using HPLC (high performance liquid chromatography). It also has been used to study the digestibility of human milk oligosaccharides (HMO).

Biochem/physiol Actions

α-amylase catalyses the hydrolysis of α-1,4 glycosidic linkage in oligosaccharides. It plays a crucial role in the initial digestion of starch, glycogen and polysaccharides. Elevated levels of amylase in serum is linked to acute pancreatitis.

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

Physical form

Lyophilized powder containing (NH4)2SO4 and sodium citrate

Preparation Note

Chromatographically purified

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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HPLC method for measurement of human salivary alpha-amylase inhibition by aqueous plant extracts
Takacs I, et al.
PLoS ONE, 68(2), 127-136 (2017)
M Alberta Araújo et al.
Biomaterials, 25(13), 2687-2693 (2004-01-31)
Apart from favourable physico-chemical and mechanical properties, the most important requirement for a biodegradable polymer to be used in medical applications is its biocompatibility and the non-cytotoxicity of its degradation products. Their combined effect should assure the safe material degradation
Stefanie Lux et al.
The British journal of nutrition, 108(7), 1177-1186 (2011-12-17)
It is proven that nuts contain essential macro- and micronutrients, e.g. fatty acids, vitamins and dietary fibre (DF). Fermentation of DF by the gut microflora results in the formation of SCFA which are recognised for their chemopreventive potential, especially by
Structure of human salivary alpha-amylase crystallized in a C-centered monoclinic space group
Fisher SZ, et al.
Acta Crystallographica Section F, Structural Biology and Crystallization Communications, 62(2), 88-93 (2006)
Copy number polymorphism of the salivary amylase gene: implications in human nutrition research
Santos JL, et al.
Lifestyle Genomics, 5(3), 117-131 (2012)

Protocols

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

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