Skip to Content
Merck
All Photos(1)

Key Documents

GE17-0998-01

Heparin Sepharose 6 Fast Flow

Cytiva 17-0998-01, pack of 50 mL

Synonym(s):

Heparin Sepharose 6 Fast Flow

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

porcine heparin

packaging

pack of 50 mL

manufacturer/tradename

Cytiva 17-0998-01

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

4-13

working range

4-12

suitability

suitable for bioprocess medium

General description

Heparin Sepharose 6 Fast Flow is a well established BioProcess affinity medium designed for capture or intermediate purification steps. This medium is extensively used for industrial purification of several marketed plasma proteins.

Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.

Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand.

This medium allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins. Additional applications include DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors.

Heparin Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Heparin Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Industrial purification of antithrombin III, coagulation factors and other plasma proteins
  • Coupling chemistry gives chemically-stable ligand attachment. Stability of medium solely dictated by heparin ligand.
  • BioProcess medium supported for industrial applications and well-established in approved processes

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol, 0.05 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocols

This page shows how to use heparin in the separation of DNA binding proteins used in HiTrap Heparin HP, HiPrep 16/10 Heparin FF and Heparin Sepharose 6 Fast Flow products from Cytiva.

Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service