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About This Item
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carrier
PAA carrier
conjugate
biotin conjugate
product line
BP-Series
Assay
95% (H-NMR)
form
lyophilized powder
mol wt
~20,000 Da
manufacturer/tradename
GlycoNZ
extent of labeling
20 mol% Biotin ligand density
technique(s)
flow cytometry: suitable
glycol-bead construction: suitable
glycol-surface construction: suitable
probe type
trisaccharide
Related Categories
1 of 4
This Item | C9995 | C0871 | C7117 |
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source rabbit |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| technique(s) indirect immunofluorescence: 10-20 μg/mL using HeLa cells, western blot: 1-2 μg/mL using HEK-293T cells expressing human Cullin-4A | technique(s) western blot: 0.7-1.5 μg/mL using HEK-293T cells expressing human Cullin-4B. | technique(s) immunoprecipitation (IP): 5-10 μg using lysate of rat brain, indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells, western blot: 1-2 μg/mL using whole extract of HEK-293T cells expressing human Cullin 3 | technique(s) western blot: 0.5-1 μg/mL using whole cell extract of human colon carcinoma HCT-116 cell line and whole cell extract of mouse fibroblasts NIH3T3 cell line |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
General description
This series of glycoconjugate probes includes HOCH2CH2NH3+-salts for acidic sugars. The affinity of these probes is 102 to 105 times higher than that of the corresponding free sugars. The spacer-arm is normally –(CH2)3–, while the spacer arm for biotin is –(CH2)6–. The flexible polymer chain behaves as an additional spacer.
Used as a high molecular weight carrier, poly[N-(2-hydroxyethyl)acrylamide], abbreviated as PAA, has
low non-specific sorption, and is stable to chemical and proteolytic action.
Application
Preparation Note
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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