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C0543

Sigma-Aldrich

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane

Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture

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Synonym(s):
Type IV collagen, EHS-collagen
CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

biological source

mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)

Quality Level

type

Type IV (Miller)

product line

BioReagent

form

lyophilized powder

packaging

glass bottle of 1 vial

technique(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm2

solubility

0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

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L2020C6745C5533
vibrant-m

C5533

Collagen from human placenta

biological source

mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)

biological source

mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)

biological source

human cell culture

biological source

human placenta

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

surface coverage

6‑10 μg/cm2

surface coverage

1‑2 μg/cm2

surface coverage

6‑10 μg/cm2

surface coverage

-

Gene Information

mouse ... Col4a1(12826), Col4a2(12827), Col4a3(12828), Col4a5(12830), Col4a6(94216)

Gene Information

mouse ... Lama1(16772), Lamb1(16777), Lamc1(226519), Lamc2(16782)

Gene Information

human ... COL4A1(1282)

Gene Information

human ... COL4A1(1282), COL4A2(1284), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

-

General description

Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells.
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Application

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used:
  • in adhesion assays.
  • in hydrogel fabrication.
  • to coat cell culture plates for cell differentiation.
  • to treat round plastic coverslips for scanning electron microscopy (SEM) analysis in an interaction study between A. culbertsoni trophozoites and Schwann cells.
Recommended for use as a cell culture substratum at 6-10 μg/cm2.

Biochem/physiol Actions

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings.

Other Notes

Native collagen
We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma-type designations with recognized collagen classification types.

Quantity

0.75 mg/vial

Preparation Note

Chloroform treated.
Pepsin is not used in the preparation.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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