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C7521

Sigma-Aldrich

Collagen from human placenta

Bornstein and Traub Type IV, powder

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Synonym(s):
Collagen Type 4
CAS Number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

human placenta

Quality Level

description

Sigma Type VI

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

HIV, hepatitis B and hepatitis C, none detected

solubility

0.5 M acetic acid: 1 mg/mL

suitability

suitable for substrate for collagenase

UniProt accession no.

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

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1 of 4

This Item
C5533C7774H4417
form

powder

form

powder

form

powder

form

solution

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

HIV, hepatitis B and hepatitis C, none detected

impurities

HIV, hepatitis B and hepatitis C, none detected

impurities

HIV, hepatitis B and hepatitis C, none detected

impurities

Endotoxin, tested, HIV, hepatitis B and hepatitis C, none detected

solubility

0.5 M acetic acid: 1 mg/mL

solubility

acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)

solubility

-

solubility

-

suitability

suitable for substrate for collagenase

suitability

-

suitability

-

suitability

-

Biochem/physiol Actions

During development, collagen IV is ubiquitously distributed in BMs. During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells.

Components

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type IV collagen occurs only in the basement membranes and contains up to six genetically distinct a-chains.

Preparation Note

This is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C. It is prepared by a modification of the pepsin extraction method of Glanville, R.W., et al.

Analysis Note

An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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