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C2249

Sigma-Aldrich

Collagen Solution from human fibroblasts

3 mg/mL, sterile-filtered, BioReagent, suitable for cell culture

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NACRES:
NA.75

biological source

human fibroblasts

Quality Level

sterility

sterile-filtered

product line

BioReagent

form

solution

packaging

pkg of 20 mL

concentration

3 mg/mL

technique(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm2

foreign activity

endotoxin ≤5.0 μmole/min-mg protein

shipped in

wet ice

storage temp.

2-8°C

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This Item
C4243C2124C6745
sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

solution

form

liquid

form

liquid

form

solution

packaging

pkg of 20 mL

packaging

pkg of 100 mL, pkg of 20 mL

packaging

pkg of 50 mL

packaging

pkg of 1 mL

concentration

3 mg/mL

concentration

2.9-3.2 mg/mL

concentration

6 mg/mL

concentration

0.3 mg/mL

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

General description

Collagen constitutes to one-third of the total protein in humans. Collagen fibril is the basic component of the tissues containing collagen. It is predominantly expressed in the extracellular matrix.
Type I collagen is a major structural component of skin, bone, tendon, and other fibrous connective tissues. It differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. Type I collagen is a heterotrimer composed of two α1(I) chains and one alpha2(I) chain, which spontaneously form a triple helix scaffold at neutral pH and 37 °C.

This product is prepared from the extracellular matrix secreted by normal human fibroblasts and contains a high monomer content. In vitro cultures were prepared using intensively tested human fibroblast cells and purified using a manufacturing process following applicable aspects of cGMP. This process contains built-in, validated steps to insure inactivation of possible prion and/or viral contaminants.

Application

Purified human collagen solution is produced from neo-natal human fibroblasts that have secreted human extracellular matrix (hECM) using a propriety cell culture production system. The human fibroblast cells have undergone significant testing demonstrating safety from viruses and other adventitious agents. Collagen Solution from human fibroblasts has been used as a component of coating solution for human bone marrow chip fabrication. It has also been used in fibroblast-like synoviocytes culturing.

Biochem/physiol Actions

Collagen is an essential ingredient of connective tissue. Studies in a Chinese family show that mutation in COL1A1 (collagenase type I) is linked with type I osteogenesis imperfecta. Collagen is linked with subchondral turnover of bone, and might have potential as marker to determine the state of joint space narrowing and osteophytes in osteoarthritis.
Proteins belonging to the collagen family supports and nourishes body tissues such as tendons, cartilage, skin and sclera of the eye. Collagen type 1 is a versatile building material required for tissue elasticity and maintains its stability and strength. Collagen is also present in byssus thread of invertebrates, which helps in surface attachment. Defective collagen affects the biomechanical property of arterial tissues.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Customers Also Viewed

Frederick Grinnell
Trends in cell biology, 13(5), 264-269 (2003-05-14)
Research on fibroblast biology in three-dimensional collagen matrices offers new opportunities to understand the reciprocal and adaptive interactions that occur between cells and surrounding matrix in a tissue-like environment. Such interactions are integral to the regulation of connective tissue morphogenesis
Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis
Kjelgaard PC, et al.
Biomarkers, 20(8), 547-556 (2015)
Cecilie Kjelgaard-Petersen et al.
Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals, 20(8), 547-556 (2016-02-11)
Characterize biomarkers measuring extracellular matrix turnover of inflamed osteoarthritis synovium. Human primary fibroblast-like synoviocytes and synovial membrane explants (SMEs) treated with various cytokines and growth factors were assessed by C1M, C3M, and acMMP3 in the conditioned medium. TNFα significantly increased
Collagen: structure and mechanics, an introduction
Collagen, 1-13 (2008)
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Molecular biology of the cell, 16(11), 5070-5076 (2005-08-19)
Fibroblast-3D collagen matrix culture provides a physiologically relevant model to study cell-matrix interactions. In tissues, fibroblasts are phagocytic cells, and in culture, they have been shown to ingest both fibronectin and collagen-coated latex particles. Compared with cells on collagen-coated coverslips

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