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C8919

Sigma-Aldrich

Collagen from calf skin

Bornstein and Traub Type I, (0.1% solution in 0.1 M acetic acid), aseptically processed, BioReagent, suitable for cell culture

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CAS Number:
MDL number:
NACRES:
NA.75

biological source

bovine (calf) skin

Quality Level

sterility

aseptically processed

product line

BioReagent

form

solution (0.1% solution in 0.1 M acetic acid)

packaging

pkg of 20 mL

availability

not available in Canada (at this time; for questions or status updates, please email us at antibody.canada@sial.com)

concentration

(0.1% solution in 0.1 M acetic acid)

technique(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm2

UniProt accession no.

binding specificity

Peptide Source: Collagen

Peptide Source: Elastin

Peptide Source: Fibronectin

shipped in

wet ice

storage temp.

2-8°C

Gene Information

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This Item
C9791C4243C2124
vibrant-m

C8919

Collagen from calf skin

vibrant-m

C9791

Collagen from calf skin

vibrant-m

C4243

Collagen solution from bovine skin

vibrant-m

C2124

Collagen solution from bovine skin

biological source

bovine (calf) skin

biological source

bovine (calf) skin

biological source

bovine skin

biological source

bovine skin

sterility

aseptically processed

sterility

-

sterility

sterile-filtered

sterility

sterile-filtered

concentration

(0.1% solution in 0.1 M acetic acid)

concentration

-

concentration

2.9-3.2 mg/mL

concentration

6 mg/mL

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

solution (0.1% solution in 0.1 M acetic acid)

form

solid

form

liquid

form

liquid

General description

Collagen type I is a component of skin, bone, tendon, and other fibrous connective tissues. It consists of repeating triplet amino acids glycine, proline and hydroxyproline. Collagen is a left handed helix with three polypeptide chains. It is a component of extracellular matrix and as close to 28 types are present in bovine.

Application

Collagen from calf skin has been used:
  • for pre-coting glass slides for immunofluorescence studies
  • as a cell adhesion factor and modification of poly(vinylidene fluoride-trifluoroethylene) (P(VDF-TrFE)) films for neuron culture
  • for coating culture dishes for murine embryonic fibroblasts culture

This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.

Biochem/physiol Actions

Collagen type I on heat denaturation results in disruption of triple helix to a randomly coil. Mutations in the collagen gene are implicated in a variety of cattle diseases. It has applications in food and cosmetics. Collagen is used as a biomaterial and as a tissue scaffold in tissue engineering. Collagen type I fiber assembly is very crucial for physiological processes and cellular signaling.

Components

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.

Preparation Note

This product is a 0.1% (1mg/ml) solution of calf skin collagen 0.1 M acetic acid. The product′s appearance is turbid, milky white solution. It is recommended that dilutions of product are prepared in sterile water. Please refer to information given on the product information sheet prior to introduction of product to cells and media.

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Extracellular Collagen VI Has Prosurvival and Autophagy Instructive Properties in Mouse Fibroblasts
Castagnaro S, et al.
Frontiers in Physiology, 9(3), 1129-1129 (2018)
Culturing neurons on MEMS fabricated P (VDF-TrFE) films for implantable artificial cochlea
Shintaku H, et al.
Journal of Biomechanical Science and Engineering, 5(3), 229-235 (2010)
Vahid Serpooshan et al.
Scientific reports, 7(1), 12590-12590 (2017-10-05)
During normal lifespan, the mammalian heart undergoes limited renewal of cardiomyocytes. While the exact mechanism for this renewal remains unclear, two possibilities have been proposed: differentiated myocyte replication and progenitor/immature cell differentiation. This study aimed to characterize a population of
Collagen: a network for regenerative medicine
Pawelec KM, et al.
Journal of Material Chemistry B: Materials for Biology and Medicine, 4(40), 6484-6496 (2016)
Hedgehog antagonist cyclopamine isomerizes to less potent forms when acidified
Wilson SR, et al.
Journal of Pharmaceutical and Biomedical Analysis, 52(5), 707-713 (2010)

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