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P5493

Sigma-Aldrich

Phosphate buffered saline

10X, solution, pH 7.2-7.6 (25 °C), BioPerformance Certified, for molecular biology

Synonym(s):

PBS

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5 UNITS
$285.00
25 UNITS
$972.00

$285.00


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5 UNITS
$285.00
25 UNITS
$972.00

About This Item

MDL number:
UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25

$285.00


In StockDetails


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Product Name

Phosphate buffered saline, 10× concentrate, BioPerformance Certified, suitable for cell culture

grade

BioPerformance Certified
for molecular biology

Quality Level

100
200

sterility

0.2 μm filtered

form

solution

storage condition

(Tightly closed)

technique(s)

cell culture | mammalian: suitable
immunocytochemistry: suitable
immunofluorescence: suitable

impurities

Bioburden, tested
endotoxin, tested
≤5 ppm heavy metals (as Pb)

color

colorless

pH

7.2-7.6 (25 °C)

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This Item
P8804525186P6621
form

lyophilized powder

form

buffered aqueous glycerol solution

form

solid

form

lyophilized powder

Quality Level

300

Quality Level

200

Quality Level

100

Quality Level

300

specific activity

≥1,000 units/mg protein (Lowry)

specific activity

≥1,000 units/mg protein (Lowry)

specific activity

≥50 units/mg dry wt.

specific activity

≥200 units/mg protein

foreign activity

Phopholipase C (lecithinase) ≤1 units/mg protein

foreign activity

Phopholipase C (lecithinase) ≤1 units/mg protein, Sphingomyelinase ≤40 units/mg protein

foreign activity

-

foreign activity

-

Gene Information

Bacillus cereus E33L ... BCZK3513(3026815)

Gene Information

Bacillus cereus E33L ... BCZK3513(3026815)

Gene Information

-

Gene Information

Bacillus cereus ATCC 10987 ... plC(2749087)

Application

Phosphate buffered saline has been used:
  • as a cell wash solution in human dental pulp stem cells (DPSCs)[1]
  • for perfusion studies in dissected brain samples[2]
  • as wash solution for neurons culture cells sample preparation for immunofluorescence and immunocytochemical labelling[3]
  • as wash solution for ovary and sperm during in vitro culture and fertilization[4]

Packaging

4L package size comes in a box with a spigot for spill free dispensing.

Other Notes

When diluted to a 1X concentration, this product will yield a phosphate buffered saline solution with a phosphate buffer concentration of 0.01M and a sodium chloride concentration of 0.154M. The solution pH will be 7.4.
For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

P5493PROC:
P5493-1L-PW:
P5493-4L-PW:
P5493-VAR:
P5493-4L:
P5493-BULK:
P5493-PH:
P5493-1L:


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Distribution and Neurochemistry of the Porcine Ileocaecal Valve Projecting Sensory Neurons in the Dorsal Root Ganglia and the Influence of Lipopolysaccharide from Different Serotypes of Salmonella spp. on the Chemical Coding of DRG Neurons in the Cell Cultures
Mikolajczyk A, et al.
International Journal of Molecular Sciences, 19(9), 2551-2551 (2018)
Maturation, Fertilization and Implantation of Cattle Ova Cultured in Different Media
Saif AH, et al.
The eurasia proceedings of science, technology, engineering & mathematics., 3(9), 181-188 (2018)
Stephen J Bush et al.
G3 (Bethesda, Md.), 9(2), 359-373 (2018-12-12)
One of the most significant physiological challenges to neonatal and juvenile ruminants is the development and establishment of the rumen. Using a subset of RNA-Seq data from our high-resolution atlas of gene expression in sheep (Ovis aries) we have provided
Differences in serum steroid hormones concentrations in spontaneously hypertensive
Kozlowska A, et al.
Physiological Research (2018)
Diverse effect of BMP-2 homodimer on mesenchymal progenitors of different origin
Hrubi E, et al.
Human Cell : Official Journal of Human Cell Research Society, 31(2), 139-148 (2018)

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