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詳細
Cell Counting kit, also known as FCCK, is a fluorescent cell counting product. The amount of a fluorescent dye, calcein, produced from Calcein-AM (3′,6′-Di(O-acetyl)-2′,7′-bis [N, Nbis-(carboxymethyl) aminomethyl]-fluorescein, tetraacetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Calcein-AM is highly lipophilic due to the presence of acetoxymethyl groups in the molecule, which rapidly permeates into the cell cytoplasm through the membrane.
アプリケーション
蛍光細胞計数キット(FCCK)は、生細胞数の蛍光検出に用いられます。細胞に含まれるエステラーゼによってカルセイン-AM(3',6'-ジ(O-アセチル)-2',7'-ビス[N,N-ビス-(カルボキシメチル) アミノメチル]-フルオレセイン、テトラアセトキシメチルエステル)から産生される蛍光色素、カルセインの量は、培地中の生細胞数に直接比例します。カルセイン-AMは、分子内のアセトキシメチル基によって親油性が高くなっているため、細胞膜を通過して速やかに細胞質に透過できます。FCCKアッセイでは、放射性同位元素([3H]-チミジン取り込みアッセイなど)や可溶化手順(MTTアッセイなど)は不要です。したがって、信頼性が高く、正確な細胞増殖アッセイの結果が得られます。
特徴および利点
Cell Counting Kit has the following advantages:
- It doesn’t require any radioisotopes to function.
- No solubilization procedure is needed.
- Results are highly reproducible and accurate.
保管分類コード
10 - Combustible liquids
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
03285-BULK-F:
03285-VAR-F:
03285-1KT-BULK-F:
03285-1KT-F:
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資料
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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