C2366

Anti-K⁺/Cl^- Cotransporter (KCC2) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 別名: Anti-DEE34, Anti-EIEE34, Anti-EIG14, Anti-KCC2, Anti-hKCC2
• MDL番号: MFCD06798467
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 化学種の反応性: canine, rat
• テクニック: western blot: 0.5-2.0 μg/mL using rat brain membrane preparation
• UniProtアクセッション番号: Q63633
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: rat ... Slc12a5(171373)

詳細

詳細

Transporters and exchangers play a critical role in the generation and dissipation of action potentials in nerve cells and in the maintenance of normal cell volume. They are also involved in a variety of mechanisms pertaining to the control of neuronal growth, maturation, synaptic plasticity and neuroendocrine functions
The cation-chloride cotransporters (CCCs) are glycoproteins involved in transport of ions, including chloride, across the cell membrane without an accompanying net charge movement; thus this type of transport is driven without the direct hydrolysis of ATP. The energy for the transport is derived from the cation gradient generated by the Na⁺/K⁺/ATPase. The CCCs also play a critical role in influencing GABA- and glycine-mediated signaling. Other transporters that participate in chloride homeostasis include Na⁺-dependent and Na⁺-independent anion exchangers, which exchange chloride for HCO3^- ions
Three cotransporters belonging to this family include sodiumchloride( Na⁺/Cl^-/ ) cotransporters (NCCs), sodiumpotassium-chloride (Na⁺/K⁺/2Cl^-) cotransporters (NKCCs) and potassium-chloride (K⁺/Cl^-) cotransporters (KCCs). K⁺/Cl^- cotransporter (KCC2) is the major neuronal chloride transporter. It has been proposed to play a role in the modulation of neuronal responses to γ-aminobutyric acid (GABA). GABA-mediated fasthyperpolarizing inhibition depends on extrusion of chloride by KCC2 In conditions of neuronal damage, where neuronal excitability is increased, and in spinal cord in models of neuropathic pain, the expression of the KCC2 transporter is decreased

特異性

Anti-K⁺/Cl^- Cotransporter (KCC2) specifically recognizes K⁺/Cl^- cotransporter. Wide range of species cross-reactivity expected due to sequence homology.

Wide range of species cross-reactivity expected due to sequence homology.

免疫原

N-terminal His-tag fusion protein corresponding to residues 932-1043 of rat K⁺/Cl^- Cotransporter (KCC2).

アプリケーション

Anti-K⁺/Cl^- Cotransporter (KCC2) specifically recognizes K⁺/Cl^- cotransporter in rat brain membrane preparations by immunoblotting and Madin Darby Canine Kidney (MDCK) cells by immunocytochemistry.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)

ターゲットの説明

Anti-K⁺/Cl^- Cotransporter (KCC2) specifically recognizes K⁺/Cl^- cotransporter (KCC2). The cation-chloride cotransporters (CCCs) are glycoproteins involved in transport of ions, includingchloride, across the ce

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)