HPLC Analysis of Proteins on BIOshell A160 Peptide C18

材料

Standard

製品番号

詳細

価格

T9128

トリプシンインヒビター Glycine max（ダイズ）由来

lyophilized powder

A2327

アドレノメジュリン 52, ヒト

≥90% (HPLC), powder

R6513

リボヌクレアーゼA ウシ膵臓由来

for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

62971

リゾチームニワトリ卵白由来

powder (crystalline), 70000-140000 U/mg

S7571

スーパーオキシドジムスターゼウシ赤血球由来

lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

41235

オボアルブミン-Atto 488コンジュゲート

suitable for fluorescence

C6749

Cytochrome c equine

recombinant, expressed in E. coli

C2624

炭酸アンヒドラーゼウシ赤血球由来

≥95% (SDS-PAGE), specific activity ≥3,500 W-A units/mg protein, lyophilized powder

分析カラム

製品番号

詳細

価格

53307-U

Ascentis^® Express Peptide ES-C18, 2.7 μm HPLCカラム

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

CONDITIONS

column

BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)

mobile phase

[A] 75:25, 0.1% TFA in water: 0.08% TFA in acetonitrile; [B] 25:75, 0.1% TFA in water: 0.08% TFA in acetonitrile

gradient

held at 0% B for 0.5 min; 0 to 100% B in 7 min

flow rate

0.3 mL/min

pressure

4743 psi (327 bar)

column temp.

35 °C

detector

UV, 220 nm

injection

1 μL

sample

0.2 - 0.6 g/L in mobile phase A

詳細

法的情報

null

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Related Applications

Small Molecule HPLC Large Molecule HPLC Gas Chromatography (GC)TLC Solid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS