Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

L3287

Sigma-Aldrich

Escort™ IV Transfection Reagent

Lipid reagent for transient and stable transfection of mammalian and insect cells.

Synonym(s):

Gene delivery

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
41106502
NACRES:
NA.25

grade

for molecular biology

Quality Level

100

form

liquid (aqueous solution)

usage

1 mL sufficient for 160-500 transfections

concentration

1 mg/mL

technique(s)

transfection: suitable

storage temp.

2-8°C

Related Categories

General description

Escort™ IV is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into a wide variety of eukaryotic cell types.

Application

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 4-16 μL Escort™ IV and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. For a list of cells that have been successfully transfected using Escort™ IV, see the Transfection Reagent Selection Guide.

Features and Benefits

  • Suitable for stable and transient transfection
  • Optimized for a wide variety of cell lines
  • Low toxicity
  • Compatible with both serum and serum-free transfection protocols
  • Ideal for Sf9, Sf21 and S2 insect cells

Components

Escort™ IV formulation:
1 mg/mL total lipid in water

Note the identity of the lipids used in Escort™ IV is confidential.

Caution

Do not freeze.

Principle

A stable complex is formed when Escort™ IV is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

Legal Information

Escort is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Victoria J Hammond et al.
The Analyst, 133(1), 71-75 (2007-12-19)
Sol-gel nanoprobes, also known as Photonic Explorer for Bioanalysis with Biologically Localised Embedding (PEBBLE), capable of performing in-vitro intracellular monitoring of reactive oxygen species have been developed using a modified form of 5(6)-carboxyfluorescein diacetate. A sol-gel matrix was selected for
R'ada Massarwa et al.
Developmental cell, 12(4), 557-569 (2007-04-11)
Formation of syncytial muscle fibers involves repeated rounds of cell fusion between growing myotubes and neighboring myoblasts. We have established that Wsp, the Drosophila homolog of the WASp family of microfilament nucleation-promoting factors, is an essential facilitator of myoblast fusion
Eva Krieghoff et al.
Journal of cell science, 119(Pt 7), 1453-1463 (2006-03-24)
beta-catenin is the central signalling molecule of the canonical Wnt pathway, where it activates target genes in a complex with LEF/TCF transcription factors in the nucleus. The regulation of beta-catenin activity is thought to occur mainly on the level of
Michael Povelones et al.
PLoS pathogens, 7(4), e1002023-e1002023 (2011-05-03)
Malaria threatens half the world's population and exacts a devastating human toll. The principal malaria vector in Africa, the mosquito Anopheles gambiae, encodes 24 members of a recently identified family of leucine-rich repeat proteins named LRIMs. Two members of this
Manveen K Gupta et al.
Antioxidants & redox signaling, 8(5-6), 1081-1093 (2006-06-15)
Cardiac myocytes, upon exposure to increasing doses of norepinephrine (NE), transit from hypertrophic to apoptotic phenotype. Since reactive oxygen species (ROS) generation is attributed to both phenomena, the authors tested whether an elevation in intracellular ROS level causes such transition.
View All Related Papers

Articles

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Protocols

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

Universal Transfection Reagent Protocol

Universal Transfection Reagent enables efficient nucleic acid delivery into various cells, compatible with different cell culture conditions.

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Related Content

Transfection Reagent Selection Guide

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service