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S1452

Sigma-Aldrich

MISSION® siRNA Transfection Reagent

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Synonym(s):
MISSION® siRNA Transfection Reagent
NACRES:
NA.51

product line

MISSION®

form

aqueous solution

shipped in

wet ice

storage temp.

2-8°C

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This Item
SHC202VSHC002VSHC204V
form

aqueous solution

form

-

form

-

form

-

storage temp.

2-8°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Quality Level

200

Quality Level

200

Quality Level

100, 200

Quality Level

100, 200

General description

The MISSION® siRNA Transfection Reagent is for the transfection of siRNA to achieve ≥90% silencing efficiency for transient knockdown of eukaryotic gene expression.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.

Other Notes

Gene silencing assay: An A549 cell line stably expressing firefly luciferase was transfected in 24-well plates with 8.4 ng of 21-mer Luciferase siRNA using 2 μL of the MISSION® siRNA Transfection Reagent. Experiments were performed in triplicate in presence of serum. Cell lysates were assessed for luciferase expression 48 hours post-transfection.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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Description
Pricing

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

120.2 °F

Flash Point(C)

49 °C


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