추천 제품
생물학적 소스
mouse
Quality Level
결합
ALEXA FLUOR™ 647
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
DM1A, monoclonal
종 반응성
guinea pig, rat, porcine, human, gerbil, mouse, bovine, avian
기술
immunocytochemistry: suitable
동형
IgG1
NCBI 수납 번호
일반 설명
Tubulin alpha-1 chain (UniProt P02552) is encoded by the TUBA1C (also known as TUBA1) gene (Gene ID 429035) in Gallus gallus (Chicken) species. Microtubules are cytoskeletal filaments composed of alpha-/beta-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tube. Various post-translational modifications, including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins (MAPs) and motor proteins.
특이성
Clone DM1A recognizes α-Tubulin, MW 50-60 kDa. The epitope recognized by clone DM1A has been mapped to the C-terminal 25 amino acids.
면역원
Epitope: C-terminal 25 amino acids.
Purified chicken brain α-Tubulin. The epitope recognized by this antibody has been mapped to the C-terminal 25 amino acids (AALEKDYEEVGVDSVEGEGEEEGEE).
애플리케이션
Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 647 conjugate is an antibody against α-Tubulin for use in Immunocytochemistry.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected α-Tubulin in NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-829) is shown to be suitable also for immunocytochemistry, immunofluorescence, and Western blotting applications.
The unconjugated antibody (Cat. No. 05-829) is shown to be suitable also for immunocytochemistry, immunofluorescence, and Western blotting applications.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
품질
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected α-Tubulin in A431 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected α-Tubulin in A431 cells.
표적 설명
~50 kDa observed.
물리적 형태
Protein G Purified
Purified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
기타 정보
Concentration: Please refer to lot specific datasheet.
법적 정보
ALEXA FLUOR is a trademark of Life Technologies
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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Cell, 184(24), 5854-5868 (2021-11-26)
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David J Schodt et al.
Biomedical optics express, 14(1), 429-440 (2023-01-27)
We describe a dedicated microscope for automated sequential localization microscopy which we term Sequential Super-resolution Microscope (SeqSRM). This microscope automates precise stage stabilization on the order of 5-10 nanometers and data acquisition of all user-selected cells on a coverslip, limiting
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Nature cell biology, 24(7), 1088-1098 (2022-06-21)
A long-established strategy for transcription regulation is the tethering of transcription factors to cellular membranes. By contrast, the principal effectors of Hedgehog signalling, the GLI transcription factors, are regulated by microtubules in the primary cilium and the cytoplasm. How GLI
Luqman O Awoniyi et al.
Journal of cell science, 136(15) (2023-07-07)
Successful B cell activation, which is critical for high-affinity antibody production, is controlled by the B cell antigen receptor (BCR). However, we still lack a comprehensive protein-level view of the very dynamic multi-branched cellular events triggered by antigen binding. Here
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