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Merck
모든 사진(2)

주요 문서

05-829-AF647

Sigma-Aldrich

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 647 conjugate

clone DM1A, from mouse, ALEXA FLUOR 647

동의어(들):

Tubulin alpha-1 chain, α-Tubulin

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

결합

ALEXA FLUOR 647

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

DM1A, monoclonal

종 반응성

guinea pig, rat, porcine, human, gerbil, mouse, bovine, avian

기술

immunocytochemistry: suitable

동형

IgG1

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... TUBA1A(7846)

일반 설명

Tubulin alpha-1 chain (UniProt P02552) is encoded by the TUBA1C (also known as TUBA1) gene (Gene ID 429035) in Gallus gallus (Chicken) species. Microtubules are cytoskeletal filaments composed of alpha-/beta-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tube. Various post-translational modifications, including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins (MAPs) and motor proteins.

특이성

Clone DM1A recognizes α-Tubulin, MW 50-60 kDa. The epitope recognized by clone DM1A has been mapped to the C-terminal 25 amino acids.

면역원

Epitope: C-terminal 25 amino acids.
Purified chicken brain α-Tubulin. The epitope recognized by this antibody has been mapped to the C-terminal 25 amino acids (AALEKDYEEVGVDSVEGEGEEEGEE).

애플리케이션

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 647 conjugate is an antibody against α-Tubulin for use in Immunocytochemistry.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected α-Tubulin in NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-829) is shown to be suitable also for immunocytochemistry, immunofluorescence, and Western blotting applications.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

품질

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected α-Tubulin in A431 cells.

표적 설명

~50 kDa observed.

물리적 형태

Protein G Purified
Purified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

법적 정보

ALEXA FLUOR is a trademark of Life Technologies

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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