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MABT205

Sigma-Aldrich

Anti-α-Tubulin Antibody, clone DM1A

clone DM1A, from mouse

Synonym(s):

Tubulin alpha-1 chain

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DM1A, monoclonal

species reactivity

mouse, human

species reactivity (predicted by homology)

chicken (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TUBA1A(7846)

General description

Tubulin is one of the main components of the cytoskeleton. It is a heterodimeric structure consisting of interlocking alpha and beta chains. Like other cytoskeleton proteins, tubulin undergoes dynamic changes depending on the cellular context, which are mediated by binding and release of GDP/GTP. Tubulin proteins may also undergo post-translational modifications, and are essential to cellular transport and mitotic processes.

Immunogen

Native chicken brain microtubules.

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected α-tubulin in NIH/3T3, A431, and HeLa cells.

Immunohistochemistry Analysis: A 1:10,000 dilution from a representative lot detected α-tubulin in normal human brain tissue, human brain cancer tissue, normal rat hippocampus tissue and normal rat cerebellum tissue.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-α-Tubulin Antibody, clone DM1A is validated for use in Western Blotting, ICC, IHC for the detection of α-Tubulin.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.01 µg/mL of this antibody detected α-tubulin in 10 µg of HeLa cell lysate.

Target description

~60 kDa observed. Uniprot describes a molecular weight at ~46 kDA, but can be higher due to post-translational modification. This protein has been observed at ~55 kDa in some cell lysates (Saugstad, J. A., et al. (2002). J Neurochem. 80(6):980-988.).

Linkage

Replaces: 04-1117

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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