MilliporeSigma
All Photos(3)

Documents

T8203

Sigma-Aldrich

Anti-α-Tubulin antibody, Mouse monoclonal

enhanced validation

clone AA13, purified from hybridoma cell culture

Sign Into View Organizational & Contract Pricing

Synonym(s):
Anti-Tuba1a, Anti-Tubulin, α1A
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AA13, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

human, rat, mouse

packaging

antibody small pack of 25 μL

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~2.0 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using Neuro-2a cell extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBA1A(7846)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
T6074T7451SAB4200776
clone

AA13, monoclonal

clone

B-5-1-2, monoclonal

clone

6-11B-1, monoclonal

clone

TUB-1A2, monoclonal

Gene Information

human ... TUBA1A(7846)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Gene Information

human ... TUBA1A(7846)

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

human, rat, mouse

species reactivity

mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat

species reactivity

bovine, frog, invertebrates, human, hamster, mouse, protista, pig, monkey, chicken, rat, plant

species reactivity

Xenopus, Arabidopsis sp., yeast, insect, rat, hamster, porcine, monkey, bovine, human, mouse, canine, chicken

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Anti-α-Tubulin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma AA13 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with rat brain tubulin.
Tubulin α 1a (TUBA1A) is a member of α-tubulin gene family. It is encoded by the gene mapped to human chromosome 12q13.12. Tubulin α 1a is a vital structural subunit of microtubules that are temporarily expressed in almost all post-mitotic neurons during neuronal development.
The encoded protein structure contains core of two β-sheets enclosed by α-helices. TUBA1A is characterized with an amino-terminal domain with the guanine nucleotide-binding region, an intermediate domain associated with interprotofilament lateral contacts and the modulation of tubulin curvature, and a carboxy-terminal domain that contains region involved in interacting with microtubule-associated proteins (MAPs) and molecular motors, such as kinesins and dynein.

Immunogen

rat brain tubulin.

Application

Anti-α-Tubulin antibody, Mouse monoclonal has been used in western blotting.
The antibody has been used in various immunochemical techniques including immunoprecipitation, immunocytochemistry, and immunohistochemistry.

Biochem/physiol Actions

Gene mutation of tubulin α-1A (TUBA1A) is observed in perisylvian asymmetrical polymicrogyria, polymicrogyria-like cortical dysplasia, and microlissencephaly in foetal cases. Alteration in the expression of the gene impairs neural migration and causes lissencephaly (LIS).
Tubulin is the major building block of microtubules, which is a structural and mobile element in mitosis, intracellular transport, flagellar movement and the cytoskeleton. Monoclonal antibodies recognizing α-tubulin, together with monoclonal antibodies to other tubulin types (α-tubulin isotypes I, II, and III, tyrosine tubulin and acetylated- α -tubulin) provide a specific and useful tool in studying the intracellular distribution of tubulin, and the static and dynamic aspects of the cytoskeleton. In addition, the various tubulin forms have been implicated in cancerous processes and are thus suggested as potential targets for cancer therapy.

Target description

Tubulin is a cylindrical, filamentous structure is present inalmost all eukaryotic cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress.
Ahlskog JK
Molecular and Cellular Biology, 30(24), 5608-5620 (2010)
New tubulin targeting agents currently in clinical development
Carlson RO
Expert Opinion on Investigational Drugs, 17(5), 707-722 (2008)
A case of TUBA1A mutation presenting with lissencephaly and Hirschsprung disease.
Brain & Development, 36(2), 159-162 (2014)
TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis.
Yokoi S
Scientific Reports, 5:15165 (2015)
Renu Srivastava et al.
The Plant cell, 25(4), 1416-1429 (2013-04-30)
BINDING PROTEIN (BiP) is a major chaperone in the endoplasmic reticulum (ER) lumen, and this study shows that BiP binds to the C-terminal tail of the stress sensor/transducer bZIP28, a membrane-associated transcription factor, retaining it in the ER under unstressed

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service