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T3526

Sigma-Aldrich

Anti-Tubulin antibody produced in rabbit

whole antiserum

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Synonym(s):
Anti-K-ALPHA-1
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

chicken, mouse

technique(s)

indirect immunofluorescence: 1:80 using cultured fibroblasts
western blot: 1:200 using chicken gizzard extract or mouse brain extract

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
T9822T6199T0198
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

rabbit

biological source

mouse

biological source

mouse

biological source

mouse

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

indirect immunofluorescence: 1:80 using cultured fibroblasts, western blot: 1:200 using chicken gizzard extract or mouse brain extract

technique(s)

immunocytochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.5-1 μg/mL using cytosolic fraction of rat brain extract

technique(s)

immunocytochemistry: 0.5-1 μg/mL using cultured chicken fibroblasts (CFB), immunohistochemistry: suitable, immunoprecipitation (IP): suitable, microarray: suitable, western blot: 0.5-1 μg/mL using total tissue extract from chicken gizzard

technique(s)

immunocytochemistry: suitable (3% paraformaldehyde-0.5% Triton X-100 and methanol-acetone), immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 0.5-1.0 μg/mL using whole extract of cultured rat adrenal pheochromocytoma (PC-12) cells

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

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General description

Microtubules are composed of tubulin and several additional proteins designated microtubule associated proteins (MAPs). Tubulin is a heterodimer which consists of an α-chain and a β-chain, both of which have a molecular weight of approximately 55,000. Microtubules form an elaborate cytoplasmic network during interphase and are reorganized into the mitotic spindle during cell division.
Tubulin is an extremely conserved and negatively charged protein. It is expressed in all eukaryotic cells, but mainly in brains.

Specificity

The product stains tubulin in cultured chicken fibroblasts or 3T3 mouse fibroblasts. Additional bands may be stained in immunoblotting.

Immunogen

microtubule proteins from brains of 13 day old chicken embryos

Application

Anti-Tubulin antibody produced in rabbit has been used in immunoblotting and indirect immunofluorescence.

Biochem/physiol Actions

αβ-tubulin heterodimers helps in formation of microtubules. Tubulin dimers exhibits GTPase activity, assembly and disassembly properties. It facilitates the construction of the flagellar apparatus.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Hong Zhu et al.
Experimental and therapeutic medicine, 15(3), 2643-2648 (2018-02-20)
The aim of the present study was to investigate the mechanism of action by which naringin reverses the resistance of ovarian cancer cells to cisplatin. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and western blotting assays were used to detect
Microtubule proteins and their post-translational forms in the cerebrospinal fluid of patients with paraparesis associated with HTLV-I infection and in SH-SY5Y cells: an in vitro model of HTLV-I-induced disease
Maldonado H, et al.
Biological Research, 41(3), 239-259 (2008)
Tubulin and microtubule structure
Downing KH and Nogales E
Current Opinion in Cell Biology, 10(1), 16-22 (1998)
Isolating tubulin from nonneural sources
Methods in Cell Biology, 95, 17-32 (2010)
Richard I Near et al.
Molecular vision, 15, 685-699 (2009-04-15)
AND-34/BCAR3 (Breast Cancer Anti-Estrogen Resistance 3) associates with the focal adhesion adaptor protein, p130CAS/BCAR1. Expression of AND-34 regulates epithelial cell growth pattern, motility, and growth factor dependence. We sought to establish the effects of the loss of AND-34 expression in

Articles

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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