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T3559

Sigma-Aldrich

Anti-γ-Tubulin antibody produced in rabbit

enhanced validation

IgG fraction of antiserum, buffered aqueous solution

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Synonym(s):
Anti-γ-Tubulin
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 48 kDa

species reactivity

human, chicken

packaging

antibody small pack of 25 μL

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 1:5,000 using methanol/acetone-fixed chicken fibroblasts
microarray: suitable
western blot: 1:1,000 using cultured A431 whole cell extracts

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

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This Item
T3320T5192T3195
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

UniProt accession no.

P23258

UniProt accession no.

P23258

UniProt accession no.

P23258

UniProt accession no.

P23258

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General description

γ-Tubulin (48kDa) is a ubiquitous and highly conserved protein within the microtubule organizing centers (MTOCs) in eukaryotic cells. It is related to α- and β-tubulin and is a member of the tubulin superfamily of proteins. γ-Tubulin abundance is less than 1% of the level of either α- or β-tubulin. γ-Tubulin shares approximately 28-32% identity with α-tubulin from various organisms and 32-36% identity with β-tubulins. Some regions are highly conserved among α-, β- and γ-tubulins.

Specificity

Anti-gamma-Tubulin recognizes an epitope located in the N-terminal region of gamma-tubulin (amino acids 38-53). Staining of gamma-tubulin (48 kDa) by immunoblotting is specifically inhibited with gamma-tubulin peptide (human, amino acids 38-53 with C-terminally added lysine).

Immunogen

synthetic peptide corresponding to the N-terminal region of human γ-tubulin (amino acids 38-53, with C-terminally added lysine) conjugated to KLH. This sequence is specific for γ-tubulin and not found in other members of the tubulin family such as α− β−, δ and ε− tubulins. This sequence is identical in mouse and rat γ-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast γ-tubulin).

Application

Anti-γ-Tubulin antibody produced in rabbit has been used in
  • immunofluorescence analysis
  • western blotting
  • immunohistochemistry

Anti-γ-Tubulin polyclonal antibody is suitable for immunoblotting of materials such as A431 cultured cells whole extract (g-tubulin, 48 kDa), and immunocytochemical staining of cells such as cultured chicken fibroblast cells.

Biochem/physiol Actions

γ-Tubulin binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. γ-Tubulin is not a member of microtubule like α- and β-tubulin. It functions as the microtubule nucleator at the microtubule organizing centre (MTOC). It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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gamma-Tubulin-gamma-Tubulin Interactions as the Basis for the Formation of a Meshwork
Rossello C, et al.
International Journal of Molecular Sciences, 19(10), 3245-3245 (2018)
Myeloproliferative disorder FOP-FGFR1 fusion kinase recruits phosphoinositide-3 kinase and phospholipase C gamma at the centrosome
Lelievre H, et al.
Molecular Cancer, 7(1), 30-30 (2008)
ATM is activated by default in mitosis, localizes at centrosomes and monitors mitotic spindle integrity
Oricchio E, et al.
Cell Cycle, 5(1), 88-92 (2006)
Ciliary abnormalities due to defects in the retrograde transport protein DYNC2H1 in short-rib polydactyly syndrome
Merrill AE, et al.
American Journal of Human Genetics, 84(4), 542-549 (2009)
Kassandra M Ori-McKenney et al.
Neuron, 76(5), 921-930 (2012-12-12)
Microtubule nucleation is essential for proper establishment and maintenance of axons and dendrites. Centrosomes, the primary site of nucleation in most cells, lose their function as microtubule organizing centers during neuronal development. How neurons generate acentrosomal microtubules remains unclear. Drosophila

Articles

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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