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04-1624

Sigma-Aldrich

Anti-Centrin Antibody, clone 20H5

clone 20H5, from mouse

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Synonym(s):
Caltractin isoform 2, EF-hand protein, calcium binding protein, centrin 1, centrin, EF-hand protein, 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

20H5, monoclonal

species reactivity

rat, human, mouse

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CETN1(1068)

General description

Centrin is a key regulator of centriole duplication. This protein is localized to the centrosome of interphase cells. During mitosis, centrin redistributes to the spindle poles, consistent with dynamic changes of the centrosome during the cell cycle. Current research suggests centrin exerts spatial and temporal control over centriole duplication through its role as a component of centriole precursor structures and through periodic cell-cycle specific changes in its abundance.

Specificity

This antibody recognizes the C-terminus of Centrin.

Immunogen

Epitope: C-terminus
Recombinant protein corresponding to the C-terminus of Chlamydomonas Centrin.

Application

Anti-Centrin Antibody, clone 20H5 is a Mouse Monoclonal Antibody for detection of Centrin also known as Caltractin isoform 2, EF-hand protein & has been validated in WB, IP, ICC.
Immunocytochemistry Analysis: Manandhar, G., et al. (1999). Cell Motil Cytoskeleton. 43(2):137-144.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected Centrin on 10 µg of HeLa cell lysate.

Target description

~ 20 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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