가격 및 재고 정보를 현재 이용할 수 없음
추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
human, mouse
기술
ELISA: suitable
immunohistochemistry: suitable (paraffin)
neutralization: suitable
western blot: suitable
동형
IgG
NCBI 수납 번호
일반 설명
Platelet-Derived Growth Factor (PDGF) is a dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB). Its receptors are transmembrane receptor tyrosine kinases, PDGF Receptors type A and B. The type A PDGF receptor binds all three PDGFs with high affinity, whereas the type B PDGF receptor is selective for PDGF-BB. PDGF signals as a growth and survival factor, as it is a potent activator of the PI3 Kinase pathway. PDGF signaling appears to play an essential role in the development of tumors of glial origin.
특이성
Reacts with PDGF-AA
면역원
Highly pure (>98%) recombinant human PDGF-AA.
애플리케이션
Detect PDGF-AA using this Anti-PDGF-AA Antibody validated for use in IH(P), ELISA, WB, NEUT.
Immunohistochemisty (paraffin): PDGF representative staining pattern/morphology in prostate cancer. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB.
ELISA: 0.5 μg/mL dilution of a previous lot allowed detection (in direct ELISA) of 0.2-0.4 ng/well when human PDGF-AA is used to coat the plate.
Neutralization: To yield ½ maiximal inhibition (ND50) of the biological activity of hPDGF-AA (5.0 ng/mL), a concentration of 0.05-0.07 μg/mL of a previous lot of this antibody was required.
Optimal working dilutions must be determined by the end user.
ELISA: 0.5 μg/mL dilution of a previous lot allowed detection (in direct ELISA) of 0.2-0.4 ng/well when human PDGF-AA is used to coat the plate.
Neutralization: To yield ½ maiximal inhibition (ND50) of the biological activity of hPDGF-AA (5.0 ng/mL), a concentration of 0.05-0.07 μg/mL of a previous lot of this antibody was required.
Optimal working dilutions must be determined by the end user.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
품질
Routinely evaluated by Western Blot on C2C12 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected PDGF-AA on 10 μg of C2C12 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected PDGF-AA on 10 μg of C2C12 lysates.
표적 설명
24 kDa detected in Western Blot.
결합
Replaces: AB1486P
물리적 형태
Affinity purified
Purified rabbit polyclonal IgG lyophilized from PBS, pH 7.2.
저장 및 안정성
The lyophilized antibody is stable at room temperature for at least one month and for up to one year when kept at -20ºC. When reconstituted in sterile water to a concentration of 0.1-1.0 mg/mL, the antibody is stable for at least six weeks at 2-8ºC.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
분석 메모
Control
C2C12 cell lysate
C2C12 cell lysate
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Activation of unfolded protein response in transgenic mouse lenses.
Reneker, LW; Chen, H; Overbeek, PA
Investigative Ophthalmology & Visual Science null
Agnieszka P Looney et al.
Bio-protocol, 9(16) (2019-09-19)
Pulmonary fibrosis is characterized by pathological scaring of the lung. Similar to other fibrotic diseases, scar formation is driven by excessive extracellular matrix deposition by activated, proliferative, and migratory fibroblasts. Currently, the two most widely used chemotaxis and cell migration
Neural grafts containing exosomes derived from Schwann cell-like cells promote peripheral nerve regeneration in rats.
Hu, et al.
Burns & trauma, 11, tkad013-tkad013 (2023)
Fibroblast-Derived STC-1 Modulates Tumor-Associated Macrophages and Lung Adenocarcinoma Development.
Tamihiro Kamata et al.
Cell reports, 31(12), 107802-107802 (2020-06-25)
The tumor microenvironment (TME) consists of different cell types, including tumor-associated macrophages (TAMs) and tumor-associated fibroblasts (TAFs). How these cells interact and contribute to lung carcinogenesis remains elusive. Using G12DKRAS- and V600EBRAF-driven mouse lung models, we identify the pleiotropic glycoprotein
Tianrun Liu et al.
Nature communications, 9(1), 3439-3439 (2018-08-29)
Angiogenesis is a hallmark of cancer. However, most malignant solid tumors exhibit robust resistance to current anti-angiogenic therapies that primarily target VEGF pathways. Here we report that endothelial-mesenchymal transformation induces glioblastoma (GBM) resistance to anti-angiogenic therapy by downregulating VEGFR-2 expression
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
