ABT146

Anti-Occludin Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Occludin
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), porcine (based on 100% sequence homology), canine (based on 100% sequence homology), primate (based on 100% sequence homology), rat (based on 100% sequence homology), equine (based on 100% sequence homology)
• 포장: antibody small pack of 25 μg
• 기술: western blot: suitable
• NCBI 수납 번호: NP_002529
• UniProt 수납 번호: Q16625
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... OCLN(4950) , OCLN(100506658)

설명

일반 설명

Occludin is a 65-kDa integral plasma-membrane protein located at the tight junctions. It is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Occludin is believed to prevent the transport of macromolecules through the tight junction. Encoded by the OLCN gene, occludin spans the plasma membrane four times, forming two extracellular loops and exposing its NH2 and COOH terminus to the cytosol. This cytoplasmic tail binds directly to the ZO family of proteins, which link the protein complex to the actin cytoskeleton. Phosphorylation/dephosphorylation plays a major role in regulation of occludin and tight junctions. Expression of occludin is required for infection of liver cells by hepatitis C virus (HCV).

특이성

This antibody recognizes the N-terminus of Occludin.

면역원

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human Occludin.

애플리케이션

Research Category
Cell Structure

Research Sub Category
ECM Proteins

This Anti-Occludin Antibody is validated for use in Western Blotting for the detection of Occludin.

품질

Evaluated by Western Blot in human kidney tissue lysate.

Western Blot Analysis: 0.2 µg/mL of this antibody detected Occludin in 10 µg of human kidney tissue lysate.

표적 설명

~63 kDa observed. The calculated molecular weight is 63 kDa, however Occludin has been shown ranging from ~55 to ~65 kDa in western blots (Florian, P., et al. (2003). Biochemical and Biophysical Research Communications. 304:5-10).

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Human kidney tissue lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.