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05-719

Sigma-Aldrich

Anti-Phosphatidylserine Antibody, clone 1H6

clone 1H6, Upstate®, from mouse

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eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1H6, monoclonal

species reactivity

vertebrates

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable

isotype

IgG

shipped in

dry ice

target post-translational modification

phosphorylation (pSer)

Gene Information

human ... PTDSS1(9791)

Related Categories

General description

Significance:
Anti-Phosphatidylserine may be used to detect translocation of the membrane phospholipid phosphatidylserine (PS) from the inner to the outer cell membrane leaflet; it provides an alternative to Annexin V for quantitation of apoptosis.
Phosphatidylserine (PS) is a phospholipid (1,2-diacyl-sn-glycerol-(3)-L-phosphoserine) found in the membrane of all plants and animals. PS is most concentrated in the brain, as PS makes up about 10% of the lipid content of neurons. Neural PS is thought to play a role in maintaining nerve cell integrity, enhancing neurotransmitter signal efficiency, and improving nerve cell signal transmission. One of the key features of apoptosis is the expresion of PS on the outer cell membrane. PS is usually found on the inner side of the plasma membrane, but during early stage apoptosis, the PS is translocated to the outer membrane. FITC-labeled annexin V can bind to the exposed PS, providing the basis of most annexin V-based apoptosis assays.

Specificity

Recognizes phosphatidylserine (PS) in cell membranes

Immunogen

Liposomes containing 70% phosphatidylserine and 30% phosphatidylglycerol

Application

Anti-Phosphatidylserine Antibody, clone 1H6 is an antibody against Phosphatidylserine for use in FC, IH.
Flow Cytometry: 0.2-2 μg/mL of a previous lot detected cell surface phosphatidylserine on Jurkat cells treated with Fas Ligand vesicles (Catalog # 01-210). See page two.

Immunohistochemistry: This antibody has been reported by an independent laboratory to detect phosphatidylserine in rat carotid artery paraffin sections fixed with ethanol/acetone (Mandinov, L., 2003).
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

routinely evaluated by flow cytometry on Jurkat cells treated with Fas Ligand vesicles (Catalog #01-210)

Target description

none given

Physical form

Format: Purified
Protein G Purified
Purified IgG supplied in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4 before the addition of glycerol to 30%. Liquid at -20°C.

Storage and Stability

Stable for 1 year at from date of receipt.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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