MilliporeSigma
All Photos(2)

P3125

Sigma-Aldrich

Papain from papaya latex

buffered aqueous suspension, 2× Crystallized, ≥16 units/mg protein

Synonym(s):
Papainase
CAS Number:
Enzyme Commission number:
MDL number:
eCl@ss:
42030311
NACRES:
NA.54

form

buffered aqueous suspension

Quality Level

quality

2× Crystallized

specific activity

≥16 units/mg protein

mol wt

23.4 kDa

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

1 of 4

This Item
P5306P33751495005
USP

USP

1495005

Papain

form

buffered aqueous suspension

form

lyophilized powder

form

crude powder

form

-

specific activity

≥16 units/mg protein

specific activity

≥8.0 units/mg protein

specific activity

1.5-10 units/mg solid

specific activity

-

mol wt

23.4 kDa

mol wt

23.4 kDa

mol wt

23.4 kDa

mol wt

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

quality

2× Crystallized

quality

-

quality

-

quality

-

General description

Papain, a cysteine protease, consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group.

Application

Papain from papaya latex has been used to digest:
  • Muller glial cells
  • cartilage explants
  • GAG and DNA content of the tissue constructs
  • decellularized tissue samples.

Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules.
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.

Biochem/physiol Actions

Papain has broad specificity, in cleaving peptide bonds of basic amino acids, leucinehydrolyzes esters and amides. Papain digests many protein substrates extensively than pancreatic proteases. Papain is commonly used in cell isolation procedures since it is more efficient and less harmful compared to other proteases. Papain significantly increases the yield of viable smooth muscle cells without affecting the cell′s sensitivity to stimulants. It catalyzes several reactions, like hydrolysis, transferase action, specificity and acyl-enzyme intermediate.
Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

Unit Definition

One unit will hydrolyze 1.0 μmole of N-α-benzoyl-L-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 °C.

Physical form

Suspension in 0.05 M sodium acetate, pH 4.5, containing 0.01% thymol

Other Notes

View more information on papain at www.sigma-aldrich.com/enzymeexplorer.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service