76216
Papain from Carica papaya
solution, light brown, ≥10 U/mg protein (~25 mg/ml)
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About This Item
Recommended Products
biological source
papaya
Quality Level
form
solution
specific activity
≥10 U/mg protein (~25 mg/ml)
mol wt
Mr ~23000
technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
color
light brown
storage temp.
2-8°C
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General description
Papain is a proteolytic enzyme and is naturally occurring in papaya (Carica papaya L.). It is obtained from the latex of cut unripe papaya. Papain belongs to the cysteine proteinase family. It is a globular, single-chain protein composed of four disulfide bridges and catalytically essential residues. Papain consists of an all α-domain and anti-parallel β-sheet domain.
Application
Papain from Carica papaya has been used:
- in the enzymatic dissociation of rat cortices to extract astrocytes for cell culture
- to study its effects on Alpaca seminal plasma viscosity and sperm function
- to study its effects on the yield and cryo-survival of Rhinoceros spermatozoa
Papain is used as a meat tenderizer. It′s also used to break up tissue in the preparation of primary cell cultures.
Biochem/physiol Actions
Papain is a cysteine protease that cleaves peptide bonds and is known to cleave the Fc portion of antibodies.
Papain plays an important role in many biological processes in many living organisms. It exhibits proteolytic activity against proteins, amino-acid esters and amide links, and short-chain peptides. Papain preferentially cleaves the peptide bonds of basic amino acids such as arginine, phenylalanine, and lysine. It has a wide range of applications in drug discovery, pharmaceutical, food, baking, and dairy industries.
Unit Definition
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-benzoyl-L-arginine ethyl ester (BAEE, Cat. No. 12880) per minute at pH 6.2 and 25°C
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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