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10108014001

Roche

Papain

from Carica papaya

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Synonym(s):
cystein protease, papain
Enzyme Commission number:

biological source

papaya

Quality Level

form

suspension

specific activity

30 U/mg
~30 units/mg protein (At 25 °C with BAEE as the substrate.)

mol wt

23 kDa

packaging

pkg of 10 mL (100 mg)

manufacturer/tradename

Roche

concentration

10-100 mg/mL

optimum pH

6.0-7.0

shipped in

wet ice

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This Item
P0609T445510108057001
Papain from Carica papaya

Roche

10108014001

Papain

TEV Protease

Sigma-Aldrich

T4455

TEV Protease

Pepsin lyophilized (salt-free), ~2500 units/mg protein (At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.)

Roche

10108057001

Pepsin

form

suspension

form

lyophilized powder

form

solution

form

lyophilized (salt-free)

specific activity

30 U/mg, ~30 units/mg protein (At 25 °C with BAEE as the substrate.)

specific activity

≥30 units/mg dry solid

specific activity

≥3,000 units/mg protein

specific activity

~2500 units/mg protein (At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.)

mol wt

23 kDa

mol wt

35 kDa

mol wt

27 kDa

mol wt

-

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Roche

concentration

10-100 mg/mL

concentration

-

concentration

-

concentration

-

General description

Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.

Application

Papain has been used in the digestion of various brain cells.
Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.

Biochem/physiol Actions

Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.

Features and Benefits

Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals

Physical form

Suspension, crystalline, nonsterile

Preparation Note

Working concentration: 0.05 to 0.5 mg/ml

Storage and Stability

Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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25G
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705578-5MG-PW

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Roche

10108057001

Pepsin

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