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B4882

Sigma-Aldrich

Bromelain from pineapple stem

≥3 units/mg protein

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CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

form

lyophilized powder

specific activity

≥3 units/mg protein

composition

Protein, ≥30% biuret

storage temp.

−20°C

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This Item
B5144C9584E0885
vibrant-m

B4882

Bromelain from pineapple stem

vibrant-m

B5144

Bromelain from pineapple stem

vibrant-m

E0885

Enterokinase from porcine intestine

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

composition

Protein, ≥30% biuret

composition

Protein, 30-50% biuret

composition

Protein, 40-70%

composition

Protein, ≥20% Lowry

Application

Bromelain from pineapple stem has been used:
  • to evaluate the aqueous micellar two-phase systems composed of ionic liquids as co-surfactants
  • as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) and in cells proliferation assay
  • to study its potential analgesic effect and to evaluate its hydrolyzingeffects on proenkephalin (PENK)-derived peptides containing enkephalin sequences

Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .

Biochem/physiol Actions

Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product B4882 is from pineapple stem. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.

Unit Definition

One unit will release 1.0 micromole of p-nitrophenol from N-alpha-CBZ-L-Lysine p-nitrophenyl ester per minute at pH 4.6 at 25 °C. One old titrimetric unit (pH 4.5 at 45 °C) is equivalent to approximately 1.7 new units (pH 4.6 at 25 °C).

Preparation Note

The product may be suspended in acetate buffer, pH 4.5 at 1 mg/mL concentration, yielding a hazy, off-white suspension.

Inhibitor

Product No.
Description
Pricing

substrate

Product No.
Description
Pricing

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Customers Also Viewed

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Enkephalin related peptides are released from jejunum wall by orally ingested bromelain
Orlandi-Mattos PE, et al.
Peptides, 115, 32-42 (2019)
In vitro antiproliferative activity of fresh pineapple juices on ovarian and colon cancer cell lines
Gani MBA, et al.
International Journal of Peptide Research and Therapeutics, 21(3), 353-364 (2015)
Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants
Vicente FA, et al.
Process. Biochem., 51(4), 528-534 (2016)
Soghra Khatun Haq et al.
European journal of biochemistry, 269(1), 47-52 (2002-01-11)
Equilibrium studies on the acid included denaturation of stem bromelain (EC 3.4.22.32) were performed by CD spectroscopy, fluorescence emission spectroscopy and binding of the hydrophobic dye, 1-anilino 8-naphthalene sulfonic acid (ANS). At pH 2.0, stem bromelain lacks a well defined
Yoriko Sawano et al.
The Journal of biological chemistry, 283(52), 36338-36343 (2008-10-25)
Bromelain isoinhibitor (bromein), a cysteine proteinase inhibitor from pineapple stem, has a unique double-chain structure. The bromein precursor protein includes three homologous inhibitor domains, each containing an interchain peptide between the light and heavy chains. The interchain peptide in the

Protocols

Protocol for HPLC Analysis of Bromelain on TSKgel® BioAssist® S vs. Competitive S Column

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