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10108057001

Roche

Pepsin

lyophilized (salt-free), ~2500 units/mg protein (At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.)

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Synonym(s):
pepsin
Enzyme Commission number:

biological source

pig gastric mucosa

Quality Level

form

lyophilized (salt-free)

specific activity

~2500 units/mg protein (At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.)

packaging

pkg of 1 g

manufacturer/tradename

Roche

optimum pH

1.8-2.2

shipped in

wet ice

storage temp.

2-8°C

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This Item
P06091141846700110127655001
Pepsin lyophilized (salt-free), ~2500 units/mg protein (At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.)

Roche

10108057001

Pepsin

form

lyophilized (salt-free)

form

lyophilized powder

form

lyophilized (salt-free)

form

suspension

specific activity

~2500 units/mg protein (At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.)

specific activity

≥30 units/mg dry solid

specific activity

≥70 units/mg protein (at 25 °C, with ATEE as a substrate)

specific activity

~350 units/mg protein (At 25 °C with glucose-6-P as the substrate.)

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

Roche

manufacturer/tradename

Roche

optimum pH

1.8-2.2

optimum pH

-

optimum pH

7.0-9.0

optimum pH

9.2

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

wet ice

General description

Pepsin is an aspartic endoproteinase isolated from pig gastric mucosa. It is mainly used for the unspecific hydrolysis of proteins and peptides in acidic media. It also provides a limited hydrolysis of native immunoglobulins to yield biologically active fragments.

Specificity

Aspartic endopeptidase with relatively broad specificity. Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1′ positions.

Application

Pepsin has been used for the digestion of liver tissue obtained from rat.

Preparation Note

Working concentration: 0.05 to 0.5 mg/ml
Storage conditions (working solution): A pepsin solution is stable at 2 to 8 °C, at least for one week, at neutral pH and under germfree conditions. Frozen aliquots of the enzyme solution are expected to be more stable.

A stock solution in 10 mM HCl (5-10 mg/ml) should be stable for at least one week at 2 to 8 °C, too. Storage light protected, tightly closed.

A short (2 minutes) incubation at 40 °C has negligible effect on the activity of lyophilized pepsin or pepsin in solution.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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