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MABT11

Sigma-Aldrich

Anti-ZO-1 Antibody, clone R40.76

clone R40.76, from rat

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Synonym(s):
tight junction protein 1, zonula occludens 1 protein, Zona occludens protein 1, Zonula occludens protein 1, zona occludens 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

R40.76, monoclonal

species reactivity

mouse

species reactivity (predicted by homology)

rat (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Tjp1(21872)

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This Item
AB2272MABT339SAB1306492
Anti-ZO-1 Antibody from rabbit, purified by affinity chromatography

Sigma-Aldrich

AB2272

Anti-ZO-1 Antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

R40.76, monoclonal

clone

polyclonal

clone

5G6.1, monoclonal

clone

polyclonal

species reactivity

mouse

species reactivity

human

species reactivity

rat, human

species reactivity

human

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

flow cytometry: 1:10-1:50, immunohistochemistry: 1:50-1:100, western blot: 1:1000

isotype

IgG2aκ

isotype

-

isotype

IgG2aκ

isotype

-

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General description

Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane associated guanylate kinase (MAGUK) family and include occludin and zona occludin family members ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.

Specificity

The antibody recognizes ZO-1.

Immunogen

Epitope: Unknown
Purified protein corresponding to mouse ZO-1.

Application

Anti-ZO-1 Antibody, clone R40.76 detects level of ZO-1 & has been published & validated for use in WB, IP, IH.
Immunofluorescence Analysis: A previous lot was used by an indepenedent laboratory in IF (D′Angelo Siliciano, 1988).
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Quality

Evaluated by Western blot in mouse brain lysate.

Western blot Analysis: 0.5 µg/mL of this antibody detected ZO-1 in 10 µg of mouse brain lysate.

Target description

~220 kDa

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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