MABE1328

Anti-INK4a (p16) Antibody, clone 13H4.1

clone 13H4.1, from mouse

• 동의어(들): Cyclin-dependent kinase inhibitor 2A, CDK4I, Multiple tumor suppressor 1, MTS-1, p16-INK4, p16-INK4a, p16INK4A
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 13H4.1, monoclonal
• 종 반응성: human
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG2bκ
• NCBI 수납 번호: NP_000068
• UniProt 수납 번호: P42771
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CDKN2A(1029)

설명

일반 설명

Cyclin-dependent kinase inhibitor 2A (UniProt P42771; also known as CDK4I, Cyclin-dependent kinase 4 inhibitor A, Multiple tumor suppressor 1, MTS-1, p16-INK4, p16-INK4a, p16INK4A) is encoded by the CDKN2A (also known as CDKN2, MTS1) gene (Gene ID 1029) in human. The INK and CIP/KIP families of cyclin dependent kinase inhibitors (CDKis) protect cells from oncogenic signals-initiated cellular transformation. INK4a and INK4b (encoded by the CDKN2A and CDKN2B gene, respectively) constitute the INK4 subfamily of CDKis. INK4a and INK4b are induced by oncogenic Ras and are involved in cell cycle G1 arrest and cellular senescence. Inactivation of CDKN2A and CDKN2B are found in a wide variety of human malignancies and murine tumor models.

특이성

Clone 13H4.1 epitope lies within a region present in p16INK4a and the spliced isoforms 2 and 5 (UniProt P42771-1, P42771-2, and P42771-4 respectively), but absent from the spliced isoform 3 (p12; UniProt P42771-3), p14ARF/p19ARF or smARF (UniProt Q8N726-1 and Q8N726-2).

면역원

GST-tagged recombinant human p16INK4a internal fragment.

애플리케이션

Anti-INK4a (p16), clone 13H4.1 Antibody, Cat. No. MABE1451, is a highly specific mouse monoclonal antibody that targets INK4a (p16) and has been tested in Immunocytochemistry and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected p16INK4a in human kidney and small intestine tissue sections.

Research Category
Epigenetics & Nuclear Function

품질

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected p16INK4a in 10 µg of HEK293 cell lysate.

표적 설명

~16 kDa observed. 16.53 kDa (isoform 1; p16INK4a), 11.22 kDa (isoform 2), and 17.88 (isoform 5; p16gamma) calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable