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Anti-p21 Antibody, clone 5G7, ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Anti-CAP20, Anti-CDKN1, Anti-CIP1, Anti-MDA-6, Anti-P21, Anti-SDI1, Anti-WAF1, Anti-p21CIP1

biological source

rabbit (recombinant)

Quality Level


expressed in HEK 293 cells



antibody form

purified antibody

antibody product type

primary antibodies


5G7, recombinant monoclonal

product line

ZooMAb® learn more



mol wt

calculated mol wt 18.12 kDa
observed mol wt ~19 kDa

species reactivity

mouse, human


antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation


immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable (peptide)



UniProt accession no.

greener alternative category

shipped in


storage temp.


target post-translational modification


Gene Information

human ... CDKN1A(1026)

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biological source

rabbit (recombinant)

biological source


biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

species reactivity

mouse, human

species reactivity

human, mouse

species reactivity

human, rat

species reactivity

mouse, human


5G7, recombinant monoclonal


recombinant monoclonal


EM05201, recombinant monoclonal


1G7, monoclonal, recombinant monoclonal

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.


Clone 5G7 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects p21/Waf1/Cip1 protein.


His-tagged full-length recombinant human Cyclin-dependent kinase inhibitor 1 (p21).


Anti-p21, clone 5G7 ZooMAb , Cat. No. ZRB1141, is a recombinant rabbit monoclonal antibody that specifically targets p21-Waf1-Cip1 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p21 in HUVEC and NIH 3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected p21 in human cervical cancer tissue sections.

Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.

Target description

Cyclin-dependent kinase inhibitor 1 (UniProt: P38936; also known as CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21) is encoded by the CDKN1A (also known as CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1) gene (Gene ID:1026) in human. p21 belongs to the Cip and Kip family of CDK inhibitors. It binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. It binds tightly to the G1 and S phase kinases, cyclin E/Cdk2, cyclin D/Cdk4, and cyclin A/Cdk2, and effectively inhibits their activity. However, its effects on G2/M kinases is minimal. It binds the cyclin subunit through a conserved Cy1 motif in the N-terminal half and through a weaker and Cy2 motif in the C-terminal half. p21 also inhibits proliferating cell nuclear antigen (PCNA) and binds to and blocks the inactivation of retinoblastoma protein (Rb), which is essential for cell cycle progression. It is an important effector of p53 and is important in the DNA-damage checkpoint. It is expressed in all adult tissues, with 5-fold lower levels observed in the brain. Is cyclin binding site is localized to amino acids 17-24 and CDK binding occurs in the region of 53-58 residues. p21 is shown to suppress tumor growth by promoting cell cycle arrest in response to various stimuli. However, it is often misregulated in various human cancers. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Abbas, T., and Dutta, A (2009). Nat Rev Cancer. 9(6); 400-414).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.


300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids




Not applicable


Not applicable

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Yogev Sela et al.
eLife, 10 (2021-02-24)
Cancer patients often harbor occult metastases, a potential source of relapse that is targetable only through systemic therapy. Studies of this occult fraction have been limited by a lack of tools with which to isolate discrete cells on spatial grounds.
Yoshihiko Kobayashi et al.
Nature cell biology, 22(8), 934-946 (2020-07-15)
Stem cells undergo dynamic changes in response to injury to regenerate lost cells. However, the identity of transitional states and the mechanisms that drive their trajectories remain understudied. Using lung organoids, multiple in vivo repair models, single-cell transcriptomics and lineage

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