COLLDISP-RO

Collagenase/Dispase^®

lyophilized, non-sterile, optimum pH 7.0-8.0

• 동의어(들): collagenase
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: enzyme from Bacillus polymyxa ( Dispase ); enzyme from bacterial ( Collagenase from Vibrio alginolyticus)
• Quality Level: 100
• 무균: non-sterile
• 양식: lyophilized
• collagenase activity: >0.1 U/mg lyophilizate, 25 °C (4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as substrate)
• 특이 활성도: >0.8 U/mg lyophilizate, pH 7.5, 37 °C (casein as substrate, Dispase)
• 포장: pkg of 100 mg (10269638001); pkg of 500 mg (11097113001)
• 제조업체/상표: Roche
• 농도: 1 mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)
• 기술: tissue processing: suitable
• 색상: light yellow to brown
• 최적 pH: 7.0-8.0
• solubility: water: soluble
• UniProt 수납 번호: P43154
• 응용 분야: life science and biopharma; sample preparation
• 외래 활성: proteolytic activities <0.1 U/mg (hemoglobin as the substrate)
• 저장 온도: 2-8°C

설명

일반 설명

Dispase is a neutral, rapid-acting, gentle protease that separates intact epidermis from dermis. It also separates cultured intact epithelial cells from the substratum. It cleaves the basement membrane zone and leaves the epithelial cells intact. Collagenase is a protease which cleaves the triple-helical protein called collagen. There are three types of tissue collagenases, and these belong to the matrix metalloproteinases (MMP) family. Collagenase obtained from Clostridium histolyticum has a very strong activity, as it digests collagen from both ends, at temperatures as low as 4-10 °C.

특이성

Collagenase degrades native collagen. Dispase^® is a nonspecific protease.

애플리케이션

Collagenase/Dispase^® is used for the preparation of cells from a wide variety of different tissues and organs.

성분

EC 3.4.24.4 & 3.4.24.3

단위 정의

One unit is the enzyme activity, which liberates under assay conditions within 1 minute Folin-positive amino acids and peptides corresponding to 1 μmol tyrosine.

제조 메모

Activator: Ca²⁺

Working concentration: Approximately 1mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)

Enzyme activity:
Collagenase: 0.1 Wünsch U/mg lyophilizate (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Dispase^®: 0.8 Wünsch U/mg lyophilizate (+37°C, casein as substrate, pH 7.5)
Contaminating proteolytic activities: <0.1U/mg lyophilizate (hemoglobin as the substrate)

Working solution: Preparation of working solution Dissolve enzyme in water to a final concentration of 100mg/mL. Dilute with PBS (Ca²⁺/Mg²⁺-free) to a final concentration of 1mg/mL and filter through a sterile 0.2μm membrane filter.
Enzyme concentration of the sterile solution: Collagenase, 0.1U/mL PBS; Dispase^®, 0.8U/mL PBS.

Inhibitors:
Collagenase inhibitors: EDTA, EGTA
Collagenase/Dispase is not inhibited by serum.

Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable at -15 to -25°C.
Note: Store in appropriate aliquots and avoid repeated freezing and thawing!

재구성

Reconstitution in water (100mg/mL). Further dilution with PBS (phosphate buffered saline) (Ca²⁺/Mg²⁺-free).

저장 및 안정성

Store dry

기타 정보

Collagenase from Vibrio alginolyticus, Dispase^® from Bacillus polymyxa

For life science research only. Not for use in diagnostic procedures.

법적 정보

Dispase is a registered trademark of Godo Shusei Co., Ltd.

안전성 정보

• 픽토그램: GHS07,GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H315 - H319 - H334 - H335
• 예방조치 성명서: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash