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SCR103

Sigma-Aldrich

Collagenase type I

The collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means.

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Synonym(s):
Collagenase Type I from Clostridium histolyticum, Tissue dissociation
eCl@ss:
32160801
NACRES:
NA.75

Quality Level

form

powder

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

shipped in

wet ice

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This Item
C1-BIOCSCR140COLLB-RO
vibrant-m

SCR103

Collagenase type I

vibrant-m

C1-BIOC

Collagenase Type I, Cls I

vibrant-m

COLLB-RO

Collagenase B

form

powder

form

powder

form

-

form

lyophilized

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

-

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Roche

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

input

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

input

-

input

-

input

-

General description

Collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means. The preparation also may contain caseinase, clostripain, and tryptic activities. This preparation can be used to dissociate cells in culture, and has been successfully used to passage human embryonic stem cells cultured with Millipore’s HEScGROTM Animal Component-Free Medium.

Packaging

1 vial containing 250mg of lyophilized powder

Quality

Each lot of collagenase type I is assayed via a modification of Mandl (1964), with the extent of collagen breakdown determined using the colorimetric ninhydrin method of Moore and Stein (1948). Each lot is then tested for successful passage of human embryonic stem cells cultured in Millipore′s HEScGRO Medium for hES cells. Cells are characterized for correct marker expression and morphology after five passages.

Physical form

Collagenase type I is supplied as a dialyzed, lyophilized powder.

Storage and Stability

Collagenase type I should be stored in its powdered form at 2-8oC until ready to use. Solutions of collagenase type I can be aliquotted and stored at -20oC, but should not be repeatedly thawed and re-frozen.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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