46950

Fluorescein 5(6)-isothiocyanate

BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

• 동의어(들): FITC, Fluorescein isothiocyanate
• 실험식(Hill 표기법): C₂₁H₁₁NO₅S
• CAS Number: 27072-45-3
• Molecular Weight: 389.38
• Beilstein: 1407295
• EC Number: 248-207-7
• MDL number: MFCD00005063
• UNSPSC 코드: 12171500
• PubChem Substance ID: 329757273
• NACRES: NA.32

속성

• Quality Level: 100
• 제품 라인: BioReagent
• 분석: ≥90% (HPLC)
• 형광: λ_ex 492 nm; λ_em 518 nm in 0.1 M phosphate pH 8.0
• 적합성: suitable for fluorescence
• 저장 온도: −20°C
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N

설명

일반 설명

Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.

애플리케이션

Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.

For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography

포장

Bottomless glass bottle. Contents are inside inserted fused cone.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Faceshields, Gloves