F7250
Fluorescein isothiocyanate isomer I
≥90% purity (HPLC), powder
Synonym(s):
FITC, Fluorescein 5-isothiocyanate
About This Item
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product name
Fluorescein isothiocyanate isomer I, suitable for protein labeling, ≥90% (HPLC), powder
Quality Level
assay
≥90% (HPLC)
form
powder
color
orange to dark orange
mp
>360 °C (lit.)
solubility
acetone: 1 mg/mL
fluorescence
λex 492 nm; λem 518 nm (green)
suitability
suitable for protein labeling
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChI key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
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General description
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.
Application
Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificates of Analysis (COA)
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