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46955

Sigma-Aldrich

Fluorescein

for fluorescence, free acid

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Synonym(s):
Acid Yellow 73
Empirical Formula (Hill Notation):
C20H12O5
CAS Number:
Molecular Weight:
332.31
Colour Index Number:
45350
Beilstein/REAXYS Number:
94324
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

grade

for fluorescence

Quality Level

technique(s)

titration: suitable

fluorescence

λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H

InChI key

GNBHRKFJIUUOQI-UHFFFAOYSA-N

Gene Information

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This Item
46960F6377F7250
vibrant-m

46955

Fluorescein

vibrant-m

46960

Fluorescein sodium salt

vibrant-m

F6377

Fluorescein sodium salt

vibrant-m

F7250

Fluorescein isothiocyanate isomer I

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

-

suitability

-

fluorescence

λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0

fluorescence

λex 460 nm; λem 515 nm(lit.), λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0

fluorescence

λex 460 nm; λem 515 nm(lit.)

fluorescence

λex 492 nm; λem 518 nm (green)

grade

for fluorescence

grade

-

grade

-

grade

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

Gene Information

human ... PTPN1(5770), PTPRF(5792)

Gene Information

-

Gene Information

-

Gene Information

-

General description

Fluorescein dye is the most commonly used fluorescent probe. It is used to stain epithelial defects and analyse the integrity of corneal epithelium. Fluorescein is used in the diagnosis of corneal perforation. Oral or intravenous fluorescein is used in fluorescein angiography to detect diabetic retinopathy and choroidal neovascular membrane.

Application

Fluorescein has been used:
  • in the permeability assay of Caco-2 cells
  • to test its suitability to serve as lifetime fluorescence standards
  • as a fluorophore for the fast recovery of fluorescence peaks and fluorophore concentration in the presence of hemoglobin
  • as a fluorescent probe in oxygen radical absorbance capacity (ORAC) assay

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Diagnostic agents
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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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