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30181

Supelco

Fluorescein sodium salt

analytical standard

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Synonym(s):
Acid Yellow 73, D&C;Yellow No. 8, NaFl, NaFluo, Sodium fluorescein, Uranine
Empirical Formula (Hill Notation):
C20H10Na2O5
CAS Number:
Molecular Weight:
376.27
Colour Index Number:
45350
Beilstein/REAXYS Number:
3833041
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.24

grade

analytical standard

Quality Level

assay

≥97.5% (HPLC)

shelf life

limited shelf life, expiry date on the label

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

impurities

≤15% water

fluorescence

λex 460 nm; λem 515 nm(lit.)

format

neat

SMILES string

[Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2

InChI key

RGPLGPBQJOQFJS-UHFFFAOYSA-L

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This Item
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Fluorescein sodium salt analytical standard

30181

Fluorescein sodium salt

Fluorescein sodium salt used as fluorescent tracer

F6377

Fluorescein sodium salt

Fluorescein sodium salt BioReagent, suitable for fluorescence

46960

Fluorescein sodium salt

Fluorescein sodium (C.I. 45350) extra pure

1.03992

Fluorescein sodium (C.I. 45350)

grade

analytical standard

grade

-

grade

-

grade

-

format

neat

format

-

format

-

format

-

assay

≥97.5% (HPLC)

assay

-

assay

-

assay

-

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

-

shelf life

-

impurities

≤15% water

impurities

-

impurities

-

impurities

-

General description

Fluorescein sodium salt (uranine) is a xanthene dye, which finds applications as a fluorescent tracer and shows the best of fluorescence in the pH range 6 to 11. Uranine is also employed as a coloring additive in drugs and cosmetics. It is known to exhibit optical chemical sensing and fluorescent properties in biological staining procedures.

Application

Fluorescein sodium salt is used as an analytical standard in determining the formation of hydroxy radicals, by the ZnO-based photocatalysts under UV-filtered simulated sunlight using spectrofluorometry. It is also used as a calibration standard for the label-free measurements of metabolic events within cells in the living animals using fluorescence lifetime imaging microscopy (FLIM).
It may also be used as an analytical reference standard for the quantification of the analyte in the following:
  • Soil samples using high-performance liquid chromatography (HPLC).
  • Coffee samples using high-performance liquid chromatography (HPLC) and capillary electrophoresis (CZE).

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Analysis Note

remarks on ICP: Na positive

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

423.7 °F - Pensky-Martens closed cup

flash_point_c

217.6 °C - Pensky-Martens closed cup


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