30181

Fluorescein sodium salt

analytical standard

• Synonym(s): Acid Yellow 73, D&C;Yellow No. 8, NaFl, NaFluo, Sodium fluorescein, Uranine
• Empirical Formula (Hill Notation): C₂₀H₁₀Na₂O₅
• CAS Number: 518-47-8
• Molecular Weight: 376.27
• Colour Index Number: 45350
• Beilstein/REAXYS Number: 3833041
• EC Number: 208-253-0
• MDL number: MFCD00167039
• UNSPSC Code: 85151701
• PubChem Substance ID: 329753341
• NACRES: NA.24

Properties

• grade: analytical standard
• Quality Level: 100
• assay: ≥97.5% (HPLC)
• shelf life: limited shelf life, expiry date on the label
• technique(s): HPLC: suitable; gas chromatography (GC): suitable
• impurities: ≤15% water
• fluorescence: λ_ex 460 nm; λ_em 515 nm(lit.)
• format: neat
• SMILES string: [Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1
• InChI: 1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2
• InChI key: RGPLGPBQJOQFJS-UHFFFAOYSA-L

Description

General description

Fluorescein sodium salt (uranine) is a xanthene dye, which finds applications as a fluorescent tracer and shows the best of fluorescence in the pH range 6 to 11. Uranine is also employed as a coloring additive in drugs and cosmetics. It is known to exhibit optical chemical sensing and fluorescent properties in biological staining procedures.

Application

Fluorescein sodium salt is used as an analytical standard in determining the formation of hydroxy radicals, by the ZnO-based photocatalysts under UV-filtered simulated sunlight using spectrofluorometry. It is also used as a calibration standard for the label-free measurements of metabolic events within cells in the living animals using fluorescence lifetime imaging microscopy (FLIM).
It may also be used as an analytical reference standard for the quantification of the analyte in the following:

• Soil samples using high-performance liquid chromatography (HPLC).
• Coffee samples using high-performance liquid chromatography (HPLC) and capillary electrophoresis (CZE).

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Analysis Note

remarks on ICP: Na positive

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: 423.7 °F - Pensky-Martens closed cup
• flash_point_c: 217.6 °C - Pensky-Martens closed cup