R6626

Rhodamine B

≥95% purity (HPLC), powder

• Synonym(s): Basic Violet 10, Brilliant Pink B, Rhodamine O, Tetraethylrhodamine
• Empirical Formula (Hill Notation): C₂₈H₃₁ClN₂O₃
• CAS Number: 81-88-9
• Molecular Weight: 479.01
• Colour Index Number: 45170
• Beilstein/REAXYS Number: 4091619
• EC Number: 201-383-9
• MDL number: MFCD00011931
• UNSPSC Code: 12171500
• PubChem Substance ID: 24899386
• NACRES: NA.47

Properties

• Product Name: Rhodamine B, ≥95% (HPLC)
• assay: ≥95% (HPLC)
• form: powder
• technique(s): titration: suitable
• color: faint green to very dark green
• mp: 210-211 (dec.) (lit.)
• solubility: H₂O: 1 mg/mL
• density: 0.79 g/cm³
• ε (extinction coefficient): 104500-115800 at 542-554 nm in methanol
• application(s): diagnostic assay manufacturing; hematology; histology

Description

General description

Rhodamine B is a xanthene dye,[1] which functions as a water tracer fluorescent. It is used as a colorant in textiles and food stuffs. Rhodamine B is associated with eye, skin and respiratory tract irritation.[2] It is used as a biomarker in oral rabies vaccine.[3]

Application

Rhodamine B has been used:

• to represent fluorescence of tetra methyl rhodamine (TMR)[4]
• in the preparation of adhesive resin for confocal laser scanning microscopy[5]
• as a fluorescent dye in intrahemolymph dye dosing and retinal image acquisition[6]
• to depict brain-specific dye capture[7]
• for imaging targets[8]

Safety Information

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H302,H318,H412
• pcodes: P264 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves