추천 제품
생물학적 소스
rabbit
Quality Level
결합
alkaline phosphatase conjugate
항체 형태
IgG fraction of antiserum
항체 생산 유형
secondary antibodies
클론
polyclonal
형태
buffered aqueous solution
종 반응성
mouse
기술
direct ELISA: 1:15,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil tissue
western blot (chemiluminescent): 1:40,000-1:80,000 using deecting β-Actin in total cell extract of HeLa cells (5-10 μg/mL)
배송 상태
wet ice
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
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일반 설명
IgG (immunoglobulin G) antibody subtype is a predominant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3.
면역원
purified mouse IgG
애플리케이션
Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in rabbit has been used in :
- direct enzyme linked immunosorbent assay (ELISA)
- immunoblotting
- immunohistochemistry
Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution.
생화학적/생리학적 작용
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG antibody participates in complement fixation and opsonization.
물리적 형태
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Guangyi Qin et al.
Oncology letters, 15(6), 8756-8760 (2018-05-29)
The present study aimed to investigate the effect of allyl isothiocyanate (AITC) on the viability and apoptosis of the human cervical cancer HeLa cell line in vitro, and to explore the potential underlying mechanisms of this. HeLa cells were treated
Structure and function of human and murine receptors for IgG
Unkeless J C, et al.
Annual Review of Immunology, 6(1), 251-281 (1988)
Dwi Susanti et al.
Frontiers in microbiology, 10, 1256-1256 (2019-07-25)
Phylogenetically deeply rooted methanogens belonging to the genus of Methanocaldococcus living in deep-sea hydrothermal vents derive energy exclusively from hydrogenotrophic methanogenesis, one of the oldest respiratory metabolisms on Earth. These hyperthermophilic, autotrophic archaea synthesize their biomolecules from inorganic substrates and
Rachel Chikwamba et al.
Transgenic research, 11(5), 479-493 (2002-11-20)
We have produced a functional heat labile enterotoxin (LT-) B subunit of Escherichia coli in maize. LT-B is a multimeric protein that presents an ideal model for an edible vaccine, displaying stability in the gut and inducing mucosal and systemic
S El-Amouri et al.
Journal of lipid research, 64(10), 100435-100435 (2023-08-29)
Obesity-associated diabetes is linked to the accumulation of ceramide in various organs, including the liver. The exact mechanisms by which ceramide contributes to diabetic pathology are unclear, but one proposed scenario is that ceramide accumulation may inhibit insulin signaling pathways.
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