MilliporeSigma
All Photos(2)

Documents

A9917

Sigma-Aldrich

Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Sign Into View Organizational & Contract Pricing

Synonym(s):
Goat Anti-Mouse IgG (Fab specific)–HRP
MDL number:
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

technique(s)

direct ELISA: 1:60,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150
western blot: 1:80,000-1:160,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
A3682A2554A2304
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

biological source

goat

biological source

goat

biological source

goat

biological source

goat

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody. IgG belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3.

Specificity

Specificity of the Anti-Mouse IgG (Fab Specific)- Peroxidase is determined by immunoelectrophoresis (IEP), prior to conjugation. By IEP, single precipitation arcs are observed against normal mouse serum, mouse IgG and the Fab fragment of mouse IgG, while no reaction is observed against the Fc fragment of mouse IgG or human IgG. Binds all mouse IgGs.

Immunogen

Purified mouse IgG Fab fragment

Application

Murine IgG levels were quantitated in brain abscess homogenates by ELISA using HRP conjugated goat anti-mouse Fab specific antibody as the detection antibody.
Anti-Mouse IgG (Fab specific)-Peroxidase antibody has been used in immunofluorescence, ELISA (enzyme-linked immunosorbent assay) and immunoblotting.
Goat Anti-Mouse IgG (Fab specific)-Peroxidase antibody can be used for western blotting assays. The product can also be used for direct ELISA (1:60,000) and IHC (1:150) applications.
Bovine CVEC and human epidermoid carcinoma A431 cell lysates were analyzed by western blot using HRP-conjugated goat anti-mouse IgG Fab specific as the secondary antibody at a 1:3000 dilution.

Biochem/physiol Actions

Immunoglobulin G (IgG) regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG1 regulates complement fixation, opsonization and antibody dependent cell mediated cytotoxicity in mice. IgG participates in hypersensitivity type II and type III.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

Preparation Note

Adsorbed to reduce background staining with human samples.
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Storage and Stability

Store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark

signalword

Warning

hcodes

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Differential binding characteristics of protein G and protein A for Fc fragments of papain-digested mouse IgG.
Aybay C
Immunology Letters, 85(3), 231-235 (2003)
Bcl-2 is a key factor for cardiac fibroblast resistance to programmed cell death.
Mayorga M, et al.
The Journal of Biological Chemistry (2004)
Silvia Aldi et al.
Glycobiology, 19(4), 337-343 (2008-11-22)
In this paper, we demonstrate the existence and localization of fucosyl-containing O-glycoforms of nucleolin in cultured bovine endothelial cells (CVEC) and malignant cultured human A431 cells. The tool for this discovery was an antibody raised against gp273, a glycoprotein ligand
P Kerr et al.
Journal of applied microbiology, 90(4), 543-549 (2001-04-20)
Production of a monoclonal antibody (MAb) to Escherichia coli O157 to develop a rapid test using a sandwich ELISA (sELISA) format. A MAb (7A6) was developed to the long-chain lipopolysaccharide of E. coli O157. A sELISA developed with the MAb
DNA immunization: ubiquitination of a viral protein enhances cytotoxic T-lymphocyte induction and antiviral protection but abrogates antibody induction.
Rodriguez F, et al.
Journal of Virology, 71(11), 8497-8503 (1997)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service