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M0659

Sigma-Aldrich

Anti-Mouse IgG (Fab specific) F(ab′)2 fragment antibody produced in goat

affinity isolated antibody

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MDL number:
NACRES:
NA.46

biological source

goat

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

concentration

2.0 mg/mL

technique(s)

indirect ELISA: suitable

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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conjugate

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conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

goat

biological source

goat

biological source

goat

biological source

sheep

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

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General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice
Anti-Mouse IgG (Fab specific) F(ab′)2 fragment antibody is monospecific as determined by immunoelectrophoresis versus normal mouse serum, mouse IgG and the Fab fragment of mouse IgG. The antibody does not react with the Fc fragment of mouse IgG. Moreover, no reactivity is observed with human κ or lambda light chain, IgG, IgA, IgM, IgD and IgE, bovine IgG and IgM, or horse IgG as determined by ELISA. In immunoelectrophoresis, the antibody preparation is found to consist only of the F(ab′)2 fragment of goat IgG, where specific antisera is applied to goat IgG fragments. No contamination with goat IgG whole molecule is observed.

Immunogen

Mouse IgG

Application

Anti-Mouse IgG (Fab specific) F(ab′)2 fragment antibody is suitable for use in ELISA (using a conc. Of 2μg/mI (100 μg/well) in 50 mM carbonate/bicarbonate buffer, at pH9.6) . The antibody can also be used in immunoelectrophoresis.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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The Ocular Conjunctiva as a Mucosal Immunization Route: A Profile of the Immune Response to the Model Antigen Tetanus Toxoid
Barisani-Asenbauer T, et al.
PLoS ONE, 8(4) (2013)
T E Steinsvik et al.
Scandinavian journal of immunology, 42(6), 607-616 (1995-12-01)
SCID and RAG-2 deficient mice were transplanted intraperitoneally with human peripheral blood lymphocytes (hu-PBL-SCID and hu-PBL-RAG mice). Seven days after transplantation the mice were immunized with a pneumococcal polysaccharide vaccine. Flow cytometry analysis of cells from the peritoneal cavity and
Priti Gupta et al.
Scientific reports, 13(1), 8850-8850 (2023-06-01)
Aging alters immunoglobulin production, affecting the humoral immune response. Toll-like receptor 2 (TLR2) recognizes Staphylococcus aureus (S. aureus) which causes bacteremia with high mortality in the elderly. To understand how TLR2 and aging affect the humoral immune response in bacteremia
Bilian Huang et al.
Frontiers in immunology, 12, 678318-678318 (2021-07-13)
Cervical cancer caused by human papillomavirus (HPV) infections is the fourth most common cancer in women worldwide. Current prophylactic HPV vaccines have achieved promising success in preventing HPV infection. However, still 570,000 new cases were reported in 2018. The current
Chen Yang et al.
Molecular medicine reports, 18(2), 1353-1360 (2018-06-15)
Previous studies have demonstrated that lipid rafts and β‑adducin serve an important role in leukocyte rolling. In the present study the migratory ability and behavior of neutrophils was demonstrated to rely on the integrity of the lipid raft structure. β‑adducin

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